Skip to main content

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (AB181832), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (AB181832), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (AB181832), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (AB181832), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Alternative names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7

Recommended products

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1620Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab181832 is the carrier-free version of Anti-Cytokeratin 7 antibody [EP1620Y] ab68460.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832), expandable thumbnail

    Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)

    This data was developed using Anti-Cytokeratin 7 antibody [EP1620Y] ab68460, the same antibody clone in a different buffer formulation.Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
    Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 & T-47D whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 in T-47D whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] (Anti-Cytokeratin 7 antibody [EP1620Y] ab68460)

    Predicted band size: 51 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)

    This data was developed using Anti-Cytokeratin 7 antibody [EP1620Y] ab68460, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform (PMID: 16265353).

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (Anti-Cytokeratin 7 antibody [EP1620Y] ab68460) at 1/20000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg/mL

    Lane 2: T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg/mL

    Lane 3: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg/mL

    Lane 4: Human placenta tissue lysate at 20 mg/mL

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 51 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)

    This data was developed using Anti-Cytokeratin 7 antibody [EP1620Y] ab68460, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (Anti-Cytokeratin 7 antibody [EP1620Y] ab68460) at 1/20000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)

    This data was developed using Anti-Cytokeratin 7 antibody [EP1620Y] ab68460, the same antibody clone in a different buffer formulation.
    Western blot: Anti-KRT7 antibody [EP1620Y] (Anti-Cytokeratin 7 antibody [EP1620Y] ab68460) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 was shown to bind specifically to KRT7. A band was observed at 40-55 kDa in wild-type A549 cell lysates with no signal observed at this size in KRT7 knockout cell line. To generate this image, wild-type and KRT7 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (Anti-Cytokeratin 7 antibody [EP1620Y] ab68460) at 1/10000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: KRT7 knockout A549 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Observed band size: 40-55 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com