Rabbit Monoclonal Cytokeratin 7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunocytochemistry-immunofluorescence-img23642.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139994.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunocytochemistry-immunofluorescence-img40336.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunocytochemistry-immunofluorescence-img12922.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (AB181831)")
Publications
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- Journal of clinical medicine 11:2022An Evaluation of the Physicochemical Properties of Preservative-Free 0.005% (/) Latanoprost Ophthalmic Solutions, and the Impact on In Vitro Human Conjunctival Goblet Cell Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosefine C Freiberg,Anne Hedengran,Steffen Heegaard,Goran Petrovski,Jette Jacobsen,Barbara Cvenkel,Miriam KolkoPubMed 35683527
- Acta ophthalmologica 100:819-8272022Generic benzalkonium chloride-preserved travoprost eye drops are not identical to the branded polyquarternium-1-preserved travoprost eye drop: Effect on cultured human conjunctival goblet cells and their physicochemical properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Hedengran,Josefine Clement Freiberg,Pernille May Hansen,Jette Jacobsen,Susan Weng Larsen,Stine Harloff-Helleberg,Kristine Freude,Gerard Boix-Lemonche,Goran Petrovski,Steffen Heegaard,Miriam KolkoPubMed 35527390
- BMJ open ophthalmology 6:e0008922021Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia Müllertz,Anne Hedengran,Zaynab Ahmad Mouhammad,Josefine Freiberg,Richárd Nagymihály,Jette Jacobsen,Susan Weng Larsen,Jeffrey Bair,Tor P Utheim,Darlene A Dartt,Steffen Heegaard,Goran Petrovski,Miriam KolkoPubMed 34993350
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
4 products for Alternative Product
6 products for Alternative Version
Target data
Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Alternative names
Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7, KRT7, SCL
Recommended products
Rabbit Monoclonal Cytokeratin 7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Alternative names
Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7, KRT7, SCL
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR1619Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab181831 is the carrier-free version of Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab68459.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunocytochemistry-immunofluorescence-img23642.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
Clone EPR1619Y (ab181831) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192077 for protocol details.
Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192077 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192077 at a working dilution of 1 in 100 (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed T47D cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139994.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Cytokeratin 7 with purified Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab68459 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab68459).
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunocytochemistry-immunofluorescence-img40336.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
Clone EPR1619Y (ab181831) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (PE). Please refer to PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab210628 for protocol details.
Figure Legend for the image: PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab210628 staining Cytokeratin 7 in T74D cells. The cells were fixed with 100%methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab210628 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in T74D cells fixed with 4%formaldehyde.
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-epr1619y-bsa-and-azide-free-ab181831--immunocytochemistry-immunofluorescence-img12922.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
Clone EPR1619Y (ab181831) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab185048 for protocol details.
Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab185048 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab185048 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed A549 cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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