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Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) is a rabbit monoclonal antibody detecting Cytokeratin 7 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 30 publications

- Trusted since 2008


Images

Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB68459), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB68459), expandable thumbnail
  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB68459), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB68459), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB68459), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/20 - 1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20 - 1/30
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Alternative names

Recommended products

Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) is a rabbit monoclonal antibody detecting Cytokeratin 7 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 30 publications

- Trusted since 2008

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1619Y
Purification technique
Affinity purification Protein A
Dissociation constant
2.1 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Cytokeratin 7?


Anti-Cytokeratin 7 [EPR1619Y] - Cytoskeleton Marker (ab68459) specifically detects a band for Cytokeratin 7 (UniProt: P08729) at a molecular weight of 51kDa.

Trusted by the scientific community


Anti-Cytokeratin 7 [EPR1619Y] - Cytoskeleton Marker (ab68459) was first used in a scientific publication in 2008 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) has been confirmed by Western blot testing in KRT7 Knockout A549 cells.



Other related products


We have a range of other formats of antibody clone [EPR1619Y] also available for your convenience:
ab68459, Carrier free - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free ab181831, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab185048, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192077, HRP - HRP Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192079, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab203427, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab203434, PE - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab210628, Carrier free - ab302460



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Lanes 1 - 4: Merged signal (red and green). Green - ab68459 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab68459 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab68459 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) at 1/5000 dilution

    Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human KRT7 knockout A549 cell line (Human KRT7 knockout A549 cell line ab261867) at 20 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Predicted band size: 51 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    ab68459 (purified) at 1/20 immunoprecipitating Cytokeratin 7 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10μg)

    Lane 2 (+): ab68459 + HeLa whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab68459 in HeLa whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunocytochemistry/Immunofluorescence analysis of T47D cells labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    Alexa Fluor® 488 (Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab185048) and Alexa Fluor® 647 (Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192077) conjugated versions are available for this clone.

  • Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) at 1/10000 dilution

    Lane 1: HeLa whole cell lysate at 20 µg

    Lane 2: SK-OV-3 whole cell lysate at 20 µg

    Lane 3: T47D whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Intracellular Flow Cytometry analysis of HeLa cells labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/20 (red). Cells were fixed with 80% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    Alexa Fluorr®488 (Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab185048) and Alexa Fluorr®647 (Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker ab192077) conjugated versions are available for this clone.

  • Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: T47D cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Cytokerain 7 with unpurified ab68459 at a dilution of 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder transitional carcinoma tissue labelling Cytokeratin 7 with unpurified ab68459.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    ab68459 showing negative staining in human sarcoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    ab68459 showing negative staining in human colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    Overlay histogram showing HeLa cells stained with unpurified ab68459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab68459, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • OI-RD Scanning - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459), expandable thumbnail

    OI-RD Scanning - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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