Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free

7 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling Cytokeratin 7 with Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Membrane and cytoplasmic staining on epithelial cells of breast tissues is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A549  (Human lung carcinoma) cells  labeling Cytokeratin 7 with Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
  The negative controls are as follows;
  1. Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
  2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).

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  Flow Cytometry (Intracellular) - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labeling Cytokeratin 7 (red) with Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at a 1/1500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Immunohistochemical analysis of frozen Mouse kidney tissue labeling Cytokeratin 7 with Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at 1/8000 dilution, followed by Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution. Cytoplasmic staining on collecting tube is observed. This data is from our collaborator Dr. Hai Song’s lab (Life Sciences Institute Zhejiang University). Counter stained with DAPI.

  Negative control: Using PBS instead of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cytokeratin 7 with Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. On mouse liver, only bile duct epithelia are positive, no reaction in hepatocytes is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Cytokeratin 7 with Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. On rat liver, only bile duct epithelia are positive, no reaction in hepatocytes is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - BSA and Azide free (ab220804)

  Cytokeratin 7 Western blot staining using rabbit Anti-Cytokeratin 7 antibody

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598).
  Western blot: Anti-KRT7 antibody [EPR17078] (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598 was shown to bind specifically to KRT7. A band was observed at 40-55 kDa in wild-type A549 cell lysates with no signal observed at this size in KRT7 knockout cell line. To generate this image, wild-type and KRT7 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker ab181598) at 1/10000 dilution

  Lane 1: Wild-type A549 cell lysate at 20 µg

  Lane 2: KRT7 knockout A549 cell lysate at 20 µg

  Lane 3: HeLa cell lysate at 20 µg

  Lane 4: MCF7 cell lysate at 20 µg

  Observed band size: 40-55 kDa