Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker

12 product images

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  Fransen-Pettersson, N. et al PLoS One. 2016 Jul 21;11(7):e0159850. doi: 10.1371/journal.pone.0159850. eCollection 2016 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Hepatic inflammation and abnormal intrahepatic bile ducts in the N-IF mouse
  

  Immunofluorescence staining of bile ducts (CK-7, red), macrophages (F4/80, light blue) and nuclei (DAPI, blue) in livers of 8 week old N-IF and 24αβNOD control mice. Representative images from two independent experiments with a total of six mice are shown. Scale bars are 100 μm.

  Cytokeratin is detected with ab181598 at1/1500 dilution in formalin-fixed, paraffin-embedded mouse liver tissue. The secondary antibody is an Anti-Rabbit AlexaFluor^®594 conjugate (red).

  (From Figure 1D of Fransen-Pettersson, N. et al)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Membrane and cytoplasmic staining on epithelial cells of breast tissues is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A549 (Human lung carcinoma) cells labeling Cytokeratin 7 with ab181598 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
  The negative controls are as follows;
  1. ab181598 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
  2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

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  Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  ~45 and 42kDa bands are supported by the literature and competitor's products, which may be the isoforms.

  All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/50000 dilution

  Lane 1: Rat bladder tissue at 10 µg

  Lane 2: Mouse lung tissue at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 51 kDa

  Observed band size: 42 kDa, 52 kDa

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  Image courtesy of Dr. Isabelle Miletich

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Immunohistochemical analysis of formaldehyde-fixed, frozen section of mouse salivary gland tissue labeling Cytokeratin 7 with ab181598 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG AlexaFluor^®660.

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  Flow Cytometry (Intracellular) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labeling Cytokeratin 7 (red) with ab181598 at a 1/1500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
  

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  Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  ~45 and 42kDa bands are supported by the literature and competitor's products, which may be the isoforms.

  All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/1000 dilution

  All lanes: Mouse liver lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 51 kDa

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Immunohistochemical analysis of frozen Mouse kidney tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution. Cytoplasmic staining on collecting tube is observed. This data is from our collaborator Dr. Hai Song’s lab (Life Sciences Institute Zhejiang University). Counter stained with DAPI.
  Negative control: Using PBS instead of primary antibody.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. On mouse liver, only bile duct epithelia are positive, no reaction in hepatocytes is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  ~45 and 42kDa bands are supported by the literature and competitor's products, which may be the isoforms.

  All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/20000 dilution

  Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

  Lane 2: Rat kidney tissue lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 51 kDa

  Observed band size: 42 kDa, 45 kDa, 51 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Cytokeratin 7 with ab181598 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. On rat liver, only bile duct epithelia are positive, no reaction in hepatocytes is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598)

  ~45 and 42kDa bands are supported by the literature and competitor's products, which may be the isoforms.

  All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (ab181598) at 1/5000 dilution

  All lanes: Mouse kidney lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 51 kDa

  Observed band size: 42 kDa, 45 kDa, 51 kDa