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Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718), expandable thumbnail
  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

-

Species
Rat
Dilution info
1/4000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/800
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells.

Alternative names

Krt2-7, Krt7, Cytokeratin-7, Keratin-7, Type-II keratin Kb7, CK-7, K7

Recommended products

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17079
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

    Lane 1: Mouse lung tissue lysate at 10 µg

    Lane 2: Mouse bladder tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa, 55 kDa

    Observed band size: 45 kDa, 51 kDa, 55 kDa

    Exposure time: 15s

  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

    All lanes: Mouse skin tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 45 kDa, 51 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718), expandable thumbnail

    Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse skin whole cell extract 10 μg (Input). Lane 2: ab199718 IP in Mouse skin whole cell extract. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199718 in Mouse skin whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    Predicted band size: 51 kDa

    Observed band size: 45 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution

    Lane 1: Rat bladder tissue lysate at 10 µg

    Lane 2: Rat lung tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 45 kDa, 51 kDa

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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