Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (AB251287)

This data was developed using ab199718, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (AB251287)

This data was developed using ab199718, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (AB251287)

This data was developed using ab199718, the same antibody clone in a different buffer formulation.

Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Mouse skin whole cell extract 10 μg (Input). Lane 2 : ab199718 IP in Mouse skin whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (<a href='/en-us/products/primary-antibodies/cytokeratin-7-antibody-epr17079-ab199718'>ab199718</a>)

Predicted band size: 51 kDa

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (AB251287)

This data was developed using ab199718, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID : 16265353.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (<a href='/en-us/products/primary-antibodies/cytokeratin-7-antibody-epr17079-ab199718'>ab199718</a>) at 1/20000 dilution

All lanes:

Mouse skin tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 45 kDa,51 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (AB251287)

This data was developed using ab199718, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID : 16265353.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (<a href='/en-us/products/primary-antibodies/cytokeratin-7-antibody-epr17079-ab199718'>ab199718</a>) at 1/4000 dilution

Lane 1:

Rat bladder tissue lysate at 10 µg

Lane 2:

Rat lung tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 45 kDa,51 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (AB251287)

This data was developed using ab199718, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID : 16265353.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (<a href='/en-us/products/primary-antibodies/cytokeratin-7-antibody-epr17079-ab199718'>ab199718</a>) at 1/20000 dilution

Lane 1:

Mouse lung tissue lysate at 10 µg

Lane 2:

Mouse bladder tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa,55 kDa

Observed band size: 45 kDa,51 kDa,55 kDa

false

Exposure time: 15s