AB216016

Anti-Cytokeratin 7 antibody [OV-TL12/30]

Name: Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016) | Abcam
Brand: Abcam Limited
SKU: AB216016
Price: 535 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Cytokeratin 7 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing KRT7 protein.

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung SCC tissue labeling Cytokeratin 7 with ab216016 at 1 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Immunocytochemistry analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab216016 followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling Cytokeratin 7 with ab216016 at 1 μg/ml.

Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab216016 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 51 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OV-TL12/30

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Cell preparation containing KRT7 protein. The exact immunogen used to generate this antibody is proprietary information.

P08729

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab216016 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:356-364 PubMed38978504

2024

Inhibition of autophagy via 3-methyladenine alleviates the progression of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Ma,Ning Ding,Lin Xie,Xiangyu Zhao,Shengchao Ma,Guizhong Li,Yinju Hao,Jiantuan Xiong,Kai Wu,Yideng Jiang,Huiping Zhang

Diagnostics (Basel, Switzerland) 13: PubMed36673009

2023

Diagnostic Algorithm for Surgical Management of Limbal Stem Cell Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Boris Malyugin,Svetlana Kalinnikova,Ruslan Isabekov,Dmitriy Ostrovskiy,Boris Knyazer,Maxim Gerasimov

iScience 25:103785 PubMed35146396

2022

PD-L1CD8 T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxia Zheng,Li Han,Zheyi Chen,Yiyang Li,Bingqian Zhou,Rui Hu,Shiyu Chen,Haibo Xiao,Yanhui Ma,Guohua Xie,Junyao Yang,Xianting Ding,Lisong Shen

European journal of pharmacology 859:172545 PubMed31319066

2019

Increased immunomodulatory capacity of human amniotic cells after activation by pro-inflammatory chemokines.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuel Kolanko,Katarzyna Kopaczka,Halina Koryciak-Komarska,Ewa Czech,Patrycja Szmytkowska,Roberto Gramignoli,Piotr Czekaj

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 7 antibody [OV-TL12/30]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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