Mouse Monoclonal Cytokeratin 7 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT7 protein.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--immunocytochemistry-immunofluorescence-img84040.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136839.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)")
![Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--flow-cytometry-img81191.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)")
![Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--western-blot-img23394.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128072.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (AB216016)")
Publications
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- European journal of pharmacology 859:1725452019Increased immunomodulatory capacity of human amniotic cells after activation by pro-inflammatory chemokines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuel Kolanko et. al.PubMed 31319066
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing KRT7 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08729
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
Associated Products
Select an associated product type
1 product for Alternative Version
7 products for Alternative Product
Target data
Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Alternative names
SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7
Recommended products
Mouse Monoclonal Cytokeratin 7 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT7 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing KRT7 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08729
- Clone number
- OV-TL12/30
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
ab216016 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--immunocytochemistry-immunofluorescence-img84040.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)
Immunocytochemistry analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab216016 followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136839.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling Cytokeratin 7 with ab216016 at 1 μg/ml.
![Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--flow-cytometry-img81191.jpg "Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)")
Flow Cytometry - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)
Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab216016 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
![Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--western-blot-img23394.jpg "Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)")
Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)
All lanes: Western blot - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 51 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-ov-tl12-30-ab216016--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128072.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [OV-TL12/30] (ab216016)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung SCC tissue labeling Cytokeratin 7 with ab216016 at 1 μg/ml.
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