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Mouse Monoclonal Cytokeratin 7 antibody. Cytoskeleton marker. Suitable for IHC-P, IHC-Fr and reacts with Human, Pig samples. Cited in 109 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human Keratin, type II cytoskeletal 7.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (AB9021), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (AB9021), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (AB9021), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (AB9021), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (AB9021), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human Keratin, type II cytoskeletal 7. Database link P08729

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-Fr
Human
Tested
Tested
Rat
Predicted
Predicted
Cat
Predicted
Predicted
Dog
Predicted
Predicted
Goat
Predicted
Predicted
Hamster
Predicted
Predicted
Pig
Expected
Tested
Zebrafish
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Goat, Hamster, Cat, Dog, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Pig

Dilution info

1/100 - 1/500

Notes

-

Species

Human

Dilution info

1/100 - 1/500

Notes

-

Predicted
Predicted

Species

Rat, Goat, Hamster, Cat, Dog, Zebrafish

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Alternative names

Recommended products

Mouse Monoclonal Cytokeratin 7 antibody. Cytoskeleton marker. Suitable for IHC-P, IHC-Fr and reacts with Human, Pig samples. Cited in 109 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human Keratin, type II cytoskeletal 7.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Native Full Length Protein corresponding to Human Keratin, type II cytoskeletal 7. Database link P08729
Clone number

RCK105

Purification technique

Affinity purification Protein G

Specificity

This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail
    Yang, X. et al PLoS One. 2013 Sep 18;8(9):e74809. doi: 10.1371/journal.pone.0074809. eCollection 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Immunofluorescent staining of β-galactosideasae and cytokeratin expression of urinary bladder after intravesical treatment with Adeno-Cre

    Formalin-fixed, paraffin-embedded mouse bladder tissue stained for β-galactosidase (green, A) or Cytokeratin 7 (red, B). Panel C shows the colocalization of β-galactosidase and Cytokeratin 7. The nuclear counterstain is DAPI (blue).

    Cytokeratin 7 was detected using ab9021.

    (After Figure 3 of Yang et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Immunohistochemistry analysis of formalin fixed, paraffin embedded sections of human liver tissue labeling Cytokeratin 7 with ab9021 at 1/100; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Immunohistochemistry analysis of frozen sections of human liver labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Immunohistochemistry analysis of frozen sections of human urinary bladder labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells and no reactivity in connective tissue.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021), expandable thumbnail
    This image is courtesy of a customer review submitted by These pictures are part of the review submitted by Todd Gocken

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

    Formalin fixed parrafin embedded mouse ovary tissue. ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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