Mouse Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Keratin, type II cytoskeletal 7 protein.
Images
![Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--flow-cytometry-img82151.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117692.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)")
![Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--protein-array-img138531.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)")
![Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--western-blot-img46226.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)")
Publications
Filter by
- Cell death discovery 8:1322022MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhao,Ge Zhao,Weiwei LiPubMed 35338123
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing Keratin, type II cytoskeletal 7 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08729
Reactivity data
Select an application| Flow Cyt | WB | Protein Array | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Alternative names
SCL, KRT7, SCL, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7
Recommended products
Mouse Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Keratin, type II cytoskeletal 7 protein.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing Keratin, type II cytoskeletal 7 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08729
- Clone number
rOV-TL12/30
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
ab218439 was purified by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--flow-cytometry-img82151.jpg "Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)")
Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab218439 at 2 μg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117692.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue labeling Cytokeratin 7 with ab218439 at 1 μg/ml.
![Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--protein-array-img138531.jpg "Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)")
Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
ab218439 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-7-antibody-rov-tl12-30-ab218439--western-blot-img46226.jpg "Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)")
Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439)
All lanes: Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439) at 2 µg/mL
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 51 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com