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AB218439

Anti-Cytokeratin 7 antibody [rOV-TL12/30]

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(2 Publications)

Mouse Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing KRT7 protein.

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7

4 Images
Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 7 with ab218439 at 2 μg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue labeling Cytokeratin 7 with ab218439 at 1 μg/ml.

Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (ab218439) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 51 kDa

false

Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)
  • Protein Array

Unknown

Protein Array - Anti-Cytokeratin 7 antibody [rOV-TL12/30] (AB218439)

ab218439 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Carrier free

    Anti-Cytokeratin 7 antibody [rOV-TL12/30] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

rOV-TL12/30

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P, Protein Array

applications

Immunogen

Cell preparation containing KRT7 protein. The exact immunogen used to generate this antibody is proprietary information.

P08729

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab218439 was purified by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
See full target information KRT7

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 64:29 PubMed38133507

2023

Isolation and Culture of Human Meibomian Gland Ductal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Peng,Ya-Li Du,Shu-Ting Liu,Hua Chen,Jia-Song Wang,Chao Wang,Hua-Tao Xie,Ming-Chang Zhang

Cell death discovery 8:132 PubMed35338123

2022

MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Ge Zhao,Weiwei Li
View all publications

Product promise

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