Anti-Cytokeratin 7 antibody [SP52]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human endometrial adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human pancreatic adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human breast tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Cytokeratin 7 with purified ab183344 at 1/100 (0.92 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human breast ductal cell carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

• WB

Lab

Western blot - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

Lanes 1 - 4 : Merged signal (red and green). Green - ab183344 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab183344 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab183344 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/25 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344) at 1/25 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

Western blot - Human KRT7 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-krt7-knockout-a549-cell-line-ab261867'>ab261867</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 51 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [SP52] (AB183344)

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344) at 1/25 dilution

All lanes:

HeLa cell lysate

Predicted band size: 51 kDa

false