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AB670

Anti-Cytokeratin 7+17 antibody [C-46]

5

(2 Reviews)

|

(3 Publications)

Mouse Monoclonal Cytokeratin 17 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing KRT7 protein.

View Alternative Names

39.1, Cytokeratin-17, Keratin-17, CK-17, K17, KRT17

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (AB670)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (AB670)

ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (AB670)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (AB670)

A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.

All lanes:

Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 2 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Non-Reducing)

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (Non-Reducing)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Reducing)

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (Reducing)

Secondary

All lanes:

IRDye800-conjugated anti-mouse IgG1 secondary antibody

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (AB670)
  • WB

Unknown

Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (AB670)

Sample preparation : Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)

All lanes:

Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/mL

All lanes:

HeLa cell lysate

Observed band size: 49 kDa,51 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-46

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Cell preparation containing KRT7 protein. The exact immunogen used to generate this antibody is proprietary information.

P08729

Specificity

Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions)</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p>pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions)</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p>pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions)</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p>pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." }, "Rabbit": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions)</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p>pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB</p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

Product details

Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from culture supernatant. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin : promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
See full target information KRT17

Additional targets

KRT7

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Psychoneuroendocrinology 120:104796 PubMed32711369

2020

Female rat sexual behavior is unaffected by perinatal fluoxetine exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Hegstad,Patty T Huijgens,Danielle J Houwing,Jocelien D A Olivier,Roy Heijkoop,Eelke M S Snoeren

Toxicology 262:162-70 PubMed19524636

2009

Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Sun,Jingbo Pi,Xueqian Wang,Erik J Tokar,Jie Liu,Michael P Waalkes

Neoplasma 37:333-42 PubMed1695327

1990

A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

B Vojtĕsek,Z Stasková,R Nenutil,J Bártková,J Kovarík,A Rejthar,J Bártek
View all publications

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