JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB41825

Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]

5

(1 Review)

|

(23 Publications)

Mouse Monoclonal Cytokeratin 19 antibody. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt, ICC/IF and reacts with Human, Rat, Pig samples. Cited in 23 publications.

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19, CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (AB41825)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (AB41825)

IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (AB41825)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (AB41825)

Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A4

Isotype

IgG1

Carrier free

No

Reacts with

Human, Pig, Rat

Applications

Flow Cyt, IHC-Fr, WB, IHC-P, ICC/IF

applications

style
left-column

Complementary Products

Assay Kits

AB252367

Human Cytokeratin 19 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cytokeratin 19 ELISA Kit (AB252367)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab91353'>ab91353</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab91353'>ab91353</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab91353'>ab91353</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Balb/c spleen cells used to produce hybridoma.

This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.3 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT19

Additional targets

KRT18
style
left-column

Publications (23)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37958530

2023

Delivery and Transcriptome Assessment of an In Vitro Three-Dimensional Proximal Tubule Model Established by Human Kidney 2 Cells in Clinical Gelatin Sponges.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yi Hsiao,Tzung-Hai Yen,Fang-Yu Wu,Chao-Min Cheng,Jia-Wei Liu,Yu-Ting Fan,Jung-Ju Huang,Chung-Yi Nien

Current biology : CB 32:4103-4113.e4 PubMed35977546

2022

High-sucrose diet exposure is associated with selective and reversible alterations in the rat peripheral taste system.

Applications

Unspecified application

Species

Unspecified reactive species

Hayeon Sung,Iva Vesela,Hannah Driks,Carrie R Ferrario,Charlotte M Mistretta,Robert M Bradley,Monica Dus

Nature 608:405-412 PubMed35922506

2022

Cellular recovery after prolonged warm ischaemia of the whole body.

Applications

Unspecified application

Species

Unspecified reactive species

David Andrijevic,Zvonimir Vrselja,Taras Lysyy,Shupei Zhang,Mario Skarica,Ana Spajic,David Dellal,Stephanie L Thorn,Robert B Duckrow,Shaojie Ma,Phan Q Duy,Atagun U Isiktas,Dan Liang,Mingfeng Li,Suel-Kee Kim,Stefano G Daniele,Khadija Banu,Sudhir Perincheri,Madhav C Menon,Anita Huttner,Kevin N Sheth,Kevin T Gobeske,Gregory T Tietjen,Hitten P Zaveri,Stephen R Latham,Albert J Sinusas,Nenad Sestan

Life (Basel, Switzerland) 11: PubMed34440558

2021

The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Snezhanna O Gening,Tatyana V Abakumova,Dina U Gafurbaeva,Albert A Rizvanov,Inna I Antoneeva,Regina R Miftakhova,Andrey B Peskov,Tatyana P Gening

Cells 9: PubMed32784507

2020

Genomic Analysis of Localized High-Risk Prostate Cancer Circulating Tumor Cells at the Single-Cell Level.

Applications

Unspecified application

Species

Unspecified reactive species

Aline Rangel-Pozzo,Songyan Liu,Gabriel Wajnberg,Xuemei Wang,Rodney J Ouellette,Geoffrey G Hicks,Darrel Drachenberg,Sabine Mai

Biomedical microdevices 21:87 PubMed31475308

2019

High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Li Hong,Man Tang,Zhengqi Chen,Zhao Ai,Feng Liu,Shuibing Wang,Nangang Zhang,Kan Liu

The oncologist 24:e1044-e1054 PubMed31300482

2019

Incorporating MicroRNA into Molecular Phenotypes of Circulating Tumor Cells Enhances the Prognostic Accuracy for Patients with Metastatic Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weige Tan,Gehao Liang,Xinhua Xie,Wenguo Jiang,Luyuan Tan,Andrew J Sanders,Zihao Liu,Yun Ling,Wenjing Zhong,Zhenluan Tian,Wanyi Lin,Chang Gong

Lung cancer (Amsterdam, Netherlands) 132:45-53 PubMed31097093

2019

Epidermal growth factor receptor-targeted immunomagnetic liposomes for circulating tumor cell enumeration in non-small cell lung cancer treated with epidermal growth factor receptor-tyrosine kinase inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Cui,Yiqian Ni,Yizhuo Zhao,Zonghai Li,Liwen Xiong,Jun Liu,Xiaofei Liang,Liyan Jiang

Nature communications 10:809 PubMed30778058

2019

MiR-135 suppresses glycolysis and promotes pancreatic cancer cell adaptation to metabolic stress by targeting phosphofructokinase-1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yang,Mari B Ishak Gabra,Eric A Hanse,Xazmin H Lowman,Thai Q Tran,Haiqing Li,Neta Milman,Juan Liu,Michael A Reid,Jason W Locasale,Ziv Gil,Mei Kong

Scientific reports 8:8751 PubMed29884810

2018

Intraoperative detection of circulating tumor cells in pulmonary venous blood during metastasectomy for colorectal lung metastases.

Applications

ICC/IF

Species

Human

Uyen-Thao Le,Peter Bronsert,Francesco Picardo,Sabine Riethdorf,Benedikt Haager,Bartosz Rylski,Martin Czerny,Friedhelm Beyersdorf,Sebastian Wiesemann,Klaus Pantel,Bernward Passlick,Jussuf Thomas Kaifi,Severin Schmid
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com