AB154301

Anti-Cytokeratin 8 antibody

Name: Anti-Cytokeratin 8 antibody (ab154301) | Abcam
Brand: Abcam Limited
SKU: AB154301
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal Cytokeratin 8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT8 aa 200 to C-terminus.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB154301)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB154301)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling Cytokeratin 8 using ab154301 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (AB154301)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (AB154301)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Cytokeratin 8 protein at cytoskeleton using ab154301 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Cytokeratin 8 antibody (AB154301)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cytokeratin 8 antibody (ab154301) at 1/20000 dilution

Lane 1:

Wild-type HeLa cell lysates at 30 µg

Lane 2:

Cytokeratin B KO HeLa cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Cytokeratin 8 antibody (AB154301)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-Cytokeratin 8 antibody (AB154301)

10% SDS PAGE

All lanes:

Western blot - Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution

All lanes:

JC cell lysate at 30 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KRT8 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05787

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7281 PubMed36435834

2022

ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Li,Qiuli Liu,Ying Han,Siyu Pei,Bisheng Cheng,Junyu Xu,Xiang Miao,Qiang Pan,Hanling Wang,Jiacheng Guo,Xuege Wang,Guoying Zhang,Yannan Lian,Wei Zhang,Yi Zang,Minjia Tan,Qintong Li,Xiaoming Wang,Yichuan Xiao,Guohong Hu,Jun Jiang,Hai Huang,Jun Qin

Tissue barriers 11:2039003 PubMed35262466

2022

GRP75 as a functional element of cholix transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keyi Liu,Tom Hunter,Alistair Taverner,Kevin Yin,Julia MacKay,Kate Colebrook,Morgan Correia,Amandine Rapp,Randall J Mrsny

Scientific reports 9:18026 PubMed31792309

2019

Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE.

Applications

Unspecified application

Species

Unspecified reactive species

Nela Pavlíková,Petr Daniel,Jan Šrámek,Michael Jelínek,Veronika Šrámková,Vlasta Němcová,Kamila Balušíková,Petr Halada,Jan Kovář

Oncotarget 10:2118-2135 PubMed31040905

2019

Microarray and pathway analysis of two COMMA-Dβ derived clones reveal important differences relevant to their developmental capacity .

Applications

Unspecified application

Species

Unspecified reactive species

Jabril R Johnson,Corinne A Boulanger,Tamaro Hudson,Evan Savage,Gilbert H Smith

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB154301)

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (AB154301)

Western blot - Anti-Cytokeratin 8 antibody (AB154301)

Western blot - Anti-Cytokeratin 8 antibody (AB154301)

Western blot - Anti-Cytokeratin 8 antibody (AB154301)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise