Rabbit Polyclonal Cytokeratin 8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT8 aa 200 to C-terminus.
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Publications
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- Nature communications 13:72812022ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNi Li,Qiuli Liu,Ying Han,Siyu Pei,Bisheng Cheng,Junyu Xu,Xiang Miao,Qiang Pan,Hanling Wang,Jiacheng Guo,Xuege Wang,Guoying Zhang,Yannan Lian,Wei Zhang,Yi Zang,Minjia Tan,Qintong Li,Xiaoming Wang,Yichuan Xiao,Guohong Hu,Jun Jiang,Hai Huang,Jun QinPubMed 36435834
- Scientific reports 9:180262019Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNela Pavlíková et. al.PubMed 31792309
- Oncotarget 10:2118-21352019Microarray and pathway analysis of two COMMA-Dβ derived clones reveal important differences relevant to their developmental capacity .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJabril R Johnson et. al.PubMed 31040905
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KRT8 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P05787
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/20000 | Notes - |
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Alternative names
CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8
Recommended products
Rabbit Polyclonal Cytokeratin 8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT8 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KRT8 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P05787
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-Cytokeratin 8 antibody (ab154301)
All lanes: Western blot - Anti-Cytokeratin 8 antibody (ab154301) at 1/20000 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: Cytokeratin 8 Knockout HeLa cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab154301)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling Cytokeratin 8 using ab154301 at 1/500 dilution.
Western blot - Anti-Cytokeratin 8 antibody (ab154301)
10% SDS PAGE
All lanes: Western blot - Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution
All lanes: JC cell lysate at 30 µg
Predicted band size: 53 kDa
Western blot - Anti-Cytokeratin 8 antibody (ab154301)
10% SDS PAGE
All lanes: Western blot - Anti-Cytokeratin 8 antibody (ab154301) at 1/10000 dilution
Lane 1: HeLa cell lysate at 30 µg
Lane 2: HepG2 cell lysate at 30 µg
Predicted band size: 53 kDa, 84 kDa
Observed band size: 100 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab154301)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Cytokeratin 8 using ab154301 at 1/500 dilution. Right panel costained with Hoechst 33342.
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