AB2530

Anti-Cytokeratin 8 antibody [C-43]

BOND RX™ Validated
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(13 Publications)

Mouse Monoclonal Cytokeratin 8 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Cell preparation containing KRT8 protein.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

ICC/IF image of ab2530 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2530, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

Overlay histogram showing MCF7 cells stained with ab2530 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2530, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

IHC image of ab2530 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2530, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

All lanes:

Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530) at 2 µg/mL

All lanes:

HeLA cell lysate

Predicted band size: 53 kDa

false

519 FITC

FITC Anti-Cytokeratin 8 antibody [C-43]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-43

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT8 protein. The exact immunogen used to generate this antibody is proprietary information.

P05787

Specificity

This antibody recognises the 52.5kDa Cytokeratin 8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.5-4 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.5-4 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.5-4 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Chicken": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.5-4 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Cow": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Hamster": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.5-4 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Pig": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Sheep": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.5-4 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from tissue culture supernatant. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of materials chemistry. B 12:8977-8992 PubMed39140678

2024

Optimization of a nanoparticle uptake protocol applied to amniotic-derived cells: unlocking the therapeutic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Peserico,Angelo Canciello,Giuseppe Prencipe,Roberto Gramignoli,Valeria Melai,Giampiero Scortichini,Mirella Bellocci,Giulia Capacchietti,Maura Turriani,Chiara Di Pancrazio,Paolo Berardinelli,Valentina Russo,Mauro Mattioli,Barbara Barboni

iScience 26:107582 PubMed37680464

2023

Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Di Lollo,Angelo Canciello,Alessia Peserico,Massimiliano Orsini,Valentina Russo,Adrián Cerveró-Varona,Beatrice Dufrusine,Mohammad El Khatib,Valentina Curini,Annunziata Mauro,Paolo Berardinelli,Cathy Tournier,Massimo Ancora,Cesare Cammà,Enrico Dainese,Luana Fiorella Mincarelli,Barbara Barboni

Cell death & disease 11:469 PubMed32555166

2020

Sodium butyrate inhibits high cholesterol-induced neuronal amyloidogenesis by modulating NRF2 stabilization-mediated ROS levels: involvement of NOX2 and SOD1.

Applications

Unspecified application

Species

Unspecified reactive species

Seo Yihl Kim,Chang Woo Chae,Hyun Jik Lee,Young Hyun Jung,Gee Euhn Choi,Jun Sung Kim,Jae Ryong Lim,Joo Eun Lee,Ji Hyeon Cho,Hansoo Park,Changho Park,Ho Jae Han

Science immunology 5: PubMed32111619

2020

PAX1 is essential for development and function of the human thymus.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Yamazaki,Raul Urrutia,Luis M Franco,Silvia Giliani,Kejian Zhang,Anas M Alazami,A Kerry Dobbs,Stefania Masneri,Avni Joshi,Francisco Otaizo-Carrasquero,Timothy G Myers,Sundar Ganesan,Maria Pia Bondioni,Mai Lan Ho,Catherine Marks,Huda Alajlan,Reem W Mohammed,Fanggeng Zou,C Alexander Valencia,Alexandra H Filipovich,Fabio Facchetti,Bertrand Boisson,Chiara Azzari,Bander K Al-Saud,Hamoud Al-Mousa,Jean Laurent Casanova,Roshini S Abraham,Luigi D Notarangelo

Stem cells international 2019:8034578 PubMed31049069

2019

In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Annunziata Mauro,Hashimita Sanyal,Angelo Canciello,Paolo Berardinelli,Valentina Russo,Nicola Bernabò,Luca Valbonetti,Barbara Barboni

Antioxidants (Basel, Switzerland) 7: PubMed30453672

2018

GPX1 Localizes to the Nucleus in Prostate Epithelium and its Levels are not Associated with Prostate Cancer Recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Dede N Ekoue,Emmanuel Ansong,Lenny K Hong,Larisa Nonn,Virgilia Macias,Ryan Deaton,Rawan Rupnow,Peter H Gann,Andre Kajdacsy-Balla,Alan M Diamond

Scientific reports 7:3761 PubMed28630448

2017

Progesterone prevents epithelial-mesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties.

Applications

IHC-P

Species

Sheep

Angelo Canciello,Valentina Russo,Paolo Berardinelli,Nicola Bernabò,Aurelio Muttini,Mauro Mattioli,Barbara Barboni

The Journal of experimental medicine 214:623-637 PubMed28148688

2017

mutations cause skeletal dysplasia, immune deficiency, and developmental delay.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Volpi,Yasuhiro Yamazaki,Patrick M Brauer,Ellen van Rooijen,Atsuko Hayashida,Anne Slavotinek,Hye Sun Kuehn,Maja Di Rocco,Carlo Rivolta,Ileana Bortolomai,Likun Du,Kerstin Felgentreff,Lisa Ott de Bruin,Kazutaka Hayashida,George Freedman,Genni Enza Marcovecchio,Kelly Capuder,Prisni Rath,Nicole Luche,Elliott J Hagedorn,Antonella Buoncompagni,Beryl Royer-Bertrand,Silvia Giliani,Pietro Luigi Poliani,Luisa Imberti,Kerry Dobbs,Fabienne E Poulain,Alberto Martini,John Manis,Robert J Linhardt,Marita Bosticardo,Sergio Damian Rosenzweig,Hane Lee,Jennifer M Puck,Juan Carlos Zúñiga-Pflücker,Leonard Zon,Pyong Woo Park,Andrea Superti-Furga,Luigi D Notarangelo

Journal of cellular biochemistry 115:1176-86 PubMed24415393

2014

Fetal liver stromal cells support blast growth in transient abnormal myelopoiesis in Down syndrome through GM-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Miyauchi,Hiroyuki Kawaguchi

Biochimie 97:210-8 PubMed24211191

2013

CARM1 and PRMT1 are dysregulated in lung cancer without hierarchical features.

Applications

Species

Human

Rania Elakoum,Guillaume Gauchotte,Abderrahim Oussalah,Marie-Pierre Wissler,Christelle Clément-Duchêne,Jean-Michel Vignaud,Jean-Louis Guéant,Farès Namour

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 antibody [C-43]

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

Western blot - Anti-Cytokeratin 8 antibody [C-43] (AB2530)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (13)

Product promise