Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (AB240986), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	ICC/IF	IHC-Fr
Human	Tested	Tested	Tested	Expected
Mouse	Expected	Expected	Expected	Tested
Rat	Expected	Expected	Expected	Tested
Pig	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Primary antibody incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Primary antibody incubation for 30 minutes at room temperature.
Species Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Associated Products

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15 products for Alternative Product

Target data

See full target information KRT8

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Alternative names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

Recommended products

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: SP102
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab240986 is the carrier-free version of Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Flow Cytometry analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1:200 dilution (0.685 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat small intestine tissue labeling Cytokeratin with Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/20 (6.85 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2 μg/ml) (Green). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. The section was incubated with Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 for 60 mins at room temperature. Confocal image showing positive staining on rat small intestine tissue. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986).
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1:25 (5.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling Cytokeratin with Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/20 (6.85 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2 μg/ml) (Green). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. The section was incubated with Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 for 60 mins at room temperature. Confocal image showing positive staining on mouse small intestine tissue. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240986).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human placenta tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free (ab240986)
Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 8 using Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 8 antibody [SP102] - C-terminal ab227644)