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AB227644

Anti-Cytokeratin 8 antibody [SP102] - C-terminal

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

17 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1 : 25 (5.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Flow Cytometry analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1 : 200 dilution (0.685 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling Cytokeratin with ab227644 at 1/20 (6.85 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab227644 for 60 mins at room temperature. Confocal image showing positive staining on mouse small intestine tissue. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by secondary antibody ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat small intestine tissue labeling Cytokeratin with ab227644 at 1/20 (6.85 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab227644 for 60 mins at room temperature. Confocal image showing positive staining on rat small intestine tissue. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by secondary antibody ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Carrier free

    Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free

  • Biotin

    Biotin Anti-Cytokeratin 8 antibody [SP102] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP102

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/20", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/20", "IHCFr-species-notes": "<p></p>" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 38:2867-2880 PubMed37565747

2023

Assessing the double-edged of extracellular signal-regulated kinase/CCAAT-enhancer-binding protein beta signaling pathway in arsenic-induced skin damage and its potential foodborne interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Dexiu Hu,Sufei Du,Liping Wu,Maoyuan Gong,Yuhong Zhang,Xingcan Yang,Yang Yang,Ruobi Chen,Yuyan Xu,Qibing Zeng

Cancer research 82:648-664 PubMed34853070

2021

Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge.

Applications

Unspecified application

Species

Unspecified reactive species

Gina Bouchard,Fernando Jose Garcia-Marques,Loukia Georgiou Karacosta,Weiruo Zhang,Abel Bermudez,Nicholas McIlvain Riley,Sushama Varma,Lindsey Catherine Mehl,Jalen Anthony Benson,Joseph B Shrager,Carolyn Ruth Bertozzi,Sharon J Pitteri,Amato J Giaccia,Sylvia Katina Plevritis
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com