Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] - BSA and Azide free (AB284762)

This data was developed using ab76584, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Cytokeratin 8 with ab76584 at 1/3600 dilution (0.035 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab76584 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] - BSA and Azide free (AB284762)

This data was developed using ab76584, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (<a href='/en-us/products/primary-antibodies/cytokeratin-8-phospho-s23-antibody-ep1629y-ab76584'>ab76584</a>) at 1/10000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HeLa treated with 100nM Calyculin A for 30mins whole cell lysate at 15 µg

Lane 3:

HeLa treated with 100nM Calyculin A for 30mins whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 7s

• Dot

Supplier Data

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] - BSA and Azide free (AB284762)

This data was developed using ab76584, the same antibody clone in a different buffer.

Dot blot analysis of Cytokeratin 8 (phospho S23) using ab76584 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100,000 dilution. Blocking and dilution buffer : 5% NFDM/TBST. Exposure time : 180s.

All lanes:

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (<a href='/en-us/products/primary-antibodies/cytokeratin-8-phospho-s23-antibody-ep1629y-ab76584'>ab76584</a>) at 1/1000 dilution

Lane 1:

Cytokeratin 8 (phospho S23) peptide

Lane 2:

EGFR non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s