Rabbit Recombinant Monoclonal antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--western-blot-img61404.gif "Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)")
![Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--immunoprecipitation-img3591.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--immunocytochemistry-immunofluorescence-img91602.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)")
Publications
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- Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing 26:297-3082021A multi-scale integrated analysis identifies KRT8 as a pan-cancer early biomarker.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMadeleine K D Scott,Maneesha Limaye,Steven Schaffert,Robert West,Michael G Ozawa,Pauline Chu,Viswam S Nair,Albert C Koong,Purvesh KhatriPubMed 33691026
- Journal of cell science 126:4721-312013A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.Applications:Unspecified applicationReactive species:Saccharomyces cerevisiae S288CTakahisa Kuga et. al.PubMed 23902688
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
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Rabbit Recombinant Monoclonal antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1630
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
Cytokeratin 8 (phospho S431) Western blot staining using rabbit Anti-Cytokeratin 8 (phospho S431) antibody
All lanes: Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) at 1/10000 dilution
Lane 1: HeLa lysates, untreated at 10 µg
Lane 2: HeLa lysates, treated with FBS and Calyculin A at 10 µg
Predicted band size: 53 kDa
Observed band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82452.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--immunoprecipitation-img3591.jpg "Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)")
Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
Purified ab109452 at 1/20 dilution (0.5μg) immunoprecipitating Cytokeratin 8 in HeLa treated with Calyculin A whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10μg
Lane 2 (+): ab109452 + HeLa treated with Calyculin A whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109452 in HeLa treated with Calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 52 kDaAll lanes: Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
Predicted band size: 53 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--immunocytochemistry-immunofluorescence-img91602.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Cytokeratin 8 with purified ab109452 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.26 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44752.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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