AB109452

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 8 phospho S431 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Cytokeratin 8 with purified ab109452 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.26 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Purified ab109452 at 1/20 dilution (0.5μg) immunoprecipitating Cytokeratin 8 in HeLa treated with Calyculin A whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10μg
Lane 2 (+) : ab109452 + HeLa treated with Calyculin A whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109452 in HeLa treated with Calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 52 kDa

All lanes:

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) at 1/10000 dilution

Lane 1:

HeLa lysates, untreated at 10 µg

Lane 2:

HeLa lysates, treated with FBS and Calyculin A at 10 µg

Predicted band size: 53 kDa

Observed band size: 52 kDa

false

Carrier free

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1630

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8 phospho S431

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:95 PubMed35080691

2022

Autophagosome-lysosome fusion is facilitated by plectin-stabilized actin and keratin 8 during macroautophagic process.

Applications

Unspecified application

Species

Unspecified reactive species

Sumin Son,Ahruem Baek,Jong Hun Lee,Dong-Eun Kim

Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing 26:297-308 PubMed33691026

2021

A multi-scale integrated analysis identifies KRT8 as a pan-cancer early biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleine K D Scott,Maneesha Limaye,Steven Schaffert,Robert West,Michael G Ozawa,Pauline Chu,Viswam S Nair,Albert C Koong,Purvesh Khatri

Journal of cell science 126:4721-31 PubMed23902688

2013

A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.

Applications

Unspecified application

Species

Saccharomyces cerevisiae S288C

Takahisa Kuga,Hideaki Kume,Naoko Kawasaki,Misako Sato,Jun Adachi,Takashi Shiromizu,Isamu Hoshino,Takanori Nishimori,Hisahiro Matsubara,Takeshi Tomonaga

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Product promise