AB284783

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Cytokeratin 8 phospho S431 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

This data was developed using ab109452, the same antibody clone in a different buffer formulation.

Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Cytokeratin 8 with purified ab109452 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.26 μg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

This data was developed using ab109452, the same antibody clone in a different buffer formulation.ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

This data was developed using ab109452, the same antibody clone in a different buffer formulation.ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

This data was developed using ab109452, the same antibody clone in a different buffer formulation.Purified ab109452 at 1/20 dilution (0.5µg) immunoprecipitating Cytokeratin 8 in HeLa treated with Calyculin A whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10µg
Lane 2 (+) : ab109452 + HeLa treated with Calyculin A whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109452 in HeLa treated with Calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 52 kDa

All lanes:

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (<a href='/en-us/products/primary-antibodies/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452'>ab109452</a>)

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

This data was developed using ab109452, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (<a href='/en-us/products/primary-antibodies/cytokeratin-8-phospho-s431-antibody-ep1630-ab109452'>ab109452</a>) at 1/10000 dilution

Lane 1:

HeLa lysates, untreated at 10 µg

Lane 2:

HeLa lysates, treated with FBS and Calyculin A at 10 µg

Predicted band size: 53 kDa

Observed band size: 52 kDa

false

Unconjugated

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1630

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Product details

ab284783 is the carrier-free version of ab109452

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8 phospho S431

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] - BSA and Azide free (AB284783)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Product promise