Anti-Cytokeratin 8 (phospho S432) antibody[RP23040156]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (AB313459)

For immunofluorescence analysis, Caco-2 cells were fixed and permeabilized for detection of endogenous Cytokeratin 8 (pS342) using ab313459 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (1 : 2000). Nuclei (blue) were stained using with DAPI, and Alexa Fluor® 555 Rhodamine Phalloidin (1 : 300) was used for cytoskeletal F-actin (red) staining. Detection and localization of Cytokeratin 8 (pS342) (green) in the cytoplasm and cytoskeleton can be clearly observed in cells treated with EGF (100 ug/ml, 20 min), as compared to untreated cells. Antibody specificity was demonstrated by competition with the Cytokeratin 8 (pS342) phosphopeptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (AB313459)

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (ab313459) at 2 µg/mL

Lane 1:

Whole cell extracts of Caco-2 at 30 µg

Lane 2:

Whole cell extracts of Caco-2 treated with EGF (100 ng/mL for 20mins) at 30 µg

Lane 3:

Whole cell extracts of A549 at 30 µg

Lane 4:

Whole cell extracts of A549 treated with EGF (100 ng/mL for 20mins) at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

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