Skip to main content

Rabbit Recombinant Multiclonal Cytokeratin 8 phospho S432 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KRT8 phospho S432.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (AB313459), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (AB313459), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human KRT8 phospho S432. Database link P05787

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
2 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Target data

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Alternative names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

Recommended products

Rabbit Recombinant Multiclonal Cytokeratin 8 phospho S432 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KRT8 phospho S432.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human KRT8 phospho S432. Database link P05787
Clone number
RP23040156
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (ab313459), expandable thumbnail

    Western blot - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (ab313459)

    All lanes: Western blot - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (ab313459) at 2 µg/mL

    Lane 1: Whole cell extracts of Caco-2 at 30 µg

    Lane 2: Whole cell extracts of Caco-2 treated with EGF (100 ng/mL for 20mins) at 30 µg

    Lane 3: Whole cell extracts of A549 at 30 µg

    Lane 4: Whole cell extracts of A549 treated with EGF (100 ng/mL for 20mins) at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (ab313459), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S432) Antibody[RP23040156] (ab313459)

    For immunofluorescence analysis, Caco-2 cells were fixed and permeabilized for detection of endogenous Cytokeratin 8 (pS342) using ab313459 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (1:2000). Nuclei (blue) were stained using with DAPI, and Alexa Fluor® 555 Rhodamine Phalloidin (1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of Cytokeratin 8 (pS342) (green) in the cytoplasm and cytoskeleton can be clearly observed in cells treated with EGF (100 ug/ml, 20 min), as compared to untreated cells. Antibody specificity was demonstrated by competition with the Cytokeratin 8 (pS342) phosphopeptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com