AB17139

Anti-Cytokeratin 8+18 antibody [5D3]

Name: Anti-Cytokeratin 8+18 antibody [5D3] (ab17139) | Abcam
Brand: Abcam Limited
SKU: AB17139
Price: 300 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(13 Publications)

Mouse Monoclonal Cytokeratin 8 antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 13 publications.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

2 Images

Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (AB17139)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (AB17139)

Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (AB17139)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (AB17139)

Paraffin embedded human skin tissue stained for Cytokeratin 8/18 using ab 17139 in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5D3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

Additional targets

KRT18

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:182 PubMed40484878

2025

Single-cell transcriptional dissection illuminates an evolution of immunosuppressive microenvironment during pancreatic ductal adenocarcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Liu,Jinen Song,Meiling Yuan,Fengli Zuo,Huihui Li,Leyi Tang,Xinmin Wang,Xueyan Wang,Qian Xiao,Li Li,Xinyu Liu,Zhankun Yang,Jianlin Wu,Jing Jing,Xuelei Ma,Hubing Shi

BMC cancer 25:989 PubMed40461968

2025

CEBPB promotes transformation of endometrial complex atypical hyperplasia to endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Tan,Lin Zhao,Daoqi Wang,Xiaodie Wu,Yongxiang Bi,Hanying Wang,Na Ma,Dehong Yang,Wei Dong,Jie Zhang

PLoS genetics 20:e1011486 PubMed39666740

2024

Loss of Notch dimerization perturbs intestinal homeostasis by a mechanism involving HDAC activity.

Applications

Unspecified application

Species

Unspecified reactive species

Quanhui Dai,Kristina Preusse,Danni Yu,Rhett A Kovall,Konrad Thorner,Xinhua Lin,Raphael Kopan

Cancer research 84:1781-1798 PubMed38507720

2024

A Subpopulation of Luminal Progenitors Secretes Pleiotrophin to Promote Angiogenesis and Metastasis in Inflammatory Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Zhang,Kaiwen Zhou,Zilin Wang,Ting Liu,Laura E Stevens,Filipa Lynce,Wendy Y Chen,Sui Peng,Yubin Xie,Duanyang Zhai,Qianjun Chen,Yawei Shi,Huijuan Shi,Zhongyu Yuan,Xiaoping Li,Juan Xu,Zhenhai Cai,Jianping Guo,Nan Shao,Ying Lin

American journal of cancer research 13:25-44 PubMed36777514

2023

Improved platform for breast cancer circulating tumor cell enrichment and characterization with next-generation sequencing technology.

Applications

Unspecified application

Species

Unspecified reactive species

Catalin Mihalcioiu,Jiarong Li,Dunarel Badescu,Anne Camirand,Nathaniel Kremer,Nicholas Bertos,Atilla Omeroglu,Michael Sebag,John Di Battista,Morag Park,Jiannis Ragoussis,Richard Kremer

Nature protocols 17:3028-3055 PubMed36180532

2022

Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D.

Applications

Unspecified application

Species

Unspecified reactive species

Ravian L van Ineveld,Raphaël Collot,Mario Barrera Román,Anna Pagliaro,Nils Bessler,Hendrikus C R Ariese,Michiel Kleinnijenhuis,Marcel Kool,Maria Alieva,Susana M Chuva de Sousa Lopes,Ellen J Wehrens,Anne C Rios

Prostate cancer and prostatic diseases 26:207-209 PubMed35058580

2022

Significant changes in macrophage and CD8 T cell densities in primary prostate tumors 2 weeks after SBRT.

Applications

Unspecified application

Species

Unspecified reactive species

Nathanael Kane,Tahmineh Romero,Silvia Diaz-Perez,Matthew B Rettig,Michael L Steinberg,Amar U Kishan,Dorthe Schaue,Robert E Reiter,Beatrice S Knudsen,Nicholas G Nickols

Hepatology (Baltimore, Md.) 74:847-863 PubMed33554350

2021

The Clinical Significance of Hepatic CD69 CD103 CD8 Resident-Memory T Cells in Autoimmune Hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrui You,You Li,Qixia Wang,Zhibin Zhao,Yikang Li,Qiwei Qian,Bo Li,Jun Zhang,Bingyuan Huang,Jubo Liang,Ruiling Chen,Zhuwan Lyu,Yong Chen,Min Lian,Xiao Xiao,Qi Miao,Jingyuan Fang,Zhexiong Lian,M Eric Gershwin,Ruqi Tang,Xiong Ma

Stem cell research & therapy 10:269 PubMed31443683

2019

Isolation and characterization of a new basal-like luminal progenitor in human breast tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Vasudeva Bhat,Victoria Lee-Wing,Pingzhao Hu,Afshin Raouf

Acta biomaterialia 88:392-405 PubMed30753941

2019

Porous Se@SiO nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-κB pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Yu Yang,Guo-Ying Deng,Rui-Zhe Zhao,Chen-Yun Dai,Chen-Yi Jiang,Xing-Jie Wang,Yi-Feng Jing,Xi-Jian Liu,Shu-Jie Xia,Bang-Min Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8+18 antibody [5D3]

Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (AB17139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (AB17139)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (13)

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