Rabbit Recombinant Monoclonal K1C9 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--western-blot-img115399.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19856.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3829.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100570.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78714.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (AB171966)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Predicted | Not recommended | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Alternative names
Cytokeratin-9, Keratin-9, CK-9, K9, KRT9
Recommended products
Rabbit Recombinant Monoclonal K1C9 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10932-62
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--western-blot-img115399.jpg "Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
All lanes: Western blot - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966) at 1/1000 dilution
Lane 1: NCI-H460 cell lysate at 10 µg
Lane 2: HL60 cell lysate at 10 µg
Lane 3: A431 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19856.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
Immunohistochemical analysis of paraffin-embedded Human skin tissue, labeling Cytokeratin 9 using ab171966 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3829.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
ab171966 showing +ve staining in Human skin tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100570.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
ab171966 showing -ve staining in Human spleen tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78714.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
ab171966 showing -ve staining in Human normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunoprecipitation-img131584.jpg "Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling Cytokeratin 9, using ab171966 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
Developed using the ECL technique.
Predicted band size: 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92173.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
ab171966 showing -ve staining in Human thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)
ab171966 showing -ve staining in Human colon adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunoprecipitation-img131584.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92173.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-9-antibody-epr10932-62-ab171966--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)")
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