AB191208

Anti-Cytokeratin antibody [34BE12]

Name: Anti-Cytokeratin antibody [34BE12] (ab191208) | Abcam
Brand: Abcam Limited
SKU: AB191208
Price: 500 USD
Availability: InStock

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(9 Publications)

Mouse Monoclonal Cytokeratin 1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (AB191208)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (AB191208)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Cytokeratin with ab191208 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (AB191208)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Cytokeratin with ab191208 at 1 μg/ml.

Supplier Data

Immunocytochemistry - Anti-Cytokeratin antibody [34BE12] (AB191208)

Immunocytochemistry analysis of MeOH-fixed HeLa (Human epithlial cell line from cervix adenocarcinoma) cells labeling Cytokeratin with ab191208 at 1 ug/ml.

Supplier Data

Western blot - Anti-Cytokeratin antibody [34BE12] (AB191208)

Western blot analysis of MCF-7 cells labeling Cytokeratin with ab191028 at 2 ug/ml.

All lanes:

Western blot - Anti-Cytokeratin antibody [34BE12] (ab191208) at 2 µg/mL

All lanes:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 66 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

34bE12

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Specificity

ab191208 recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. ab191208 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

purified from Bioreactor Concentrates by Protein A/G column chromatography

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratins also known by the abbreviation 'CK' are a class of intermediate filament proteins providing structural support to epithelial cells. Their mass ranges between 40 kDa to 68 kDa. Cytokeratins are expressed in epithelial tissues including the outer layer of skin and line organs such as the gut and respiratory tract. Commonly referenced with specific subtypes CK MNF116 or CK cocktail antibodies are used for detection in laboratory settings. These proteins help maintain cell integrity and protect from mechanical stress.

Biological function summary

Keratins function as a complex to create a stable cytoskeletal network inside epithelial cells. They interact with proteins to form keratin filaments contributing to the mechanical strength and resilience of cells. Cytokeratins also play a role in intracellular signaling and cellular differentiation. They participate in wound healing processes through modulation of cell proliferation and apoptosis. The CK MNF116 marker is especially noted for its application in identifying and characterizing epithelial proteins in pathology.

Pathways

Cytokeratins are involved in pathways related to cell structure and stress response. Specifically they play key roles in the Epidermal Growth Factor (EGF) signaling pathway and the cell cycle control pathway. These pathways involve proteins like epidermal growth factor receptors (EGFR) and cyclins which regulate cell cycle progression. Cytokeratins influence these pathways by impacting cell adhesion and proliferation essential for maintaining epithelial cell functions.

Cytokeratins have significant roles in cancer diagnostics particularly in epithelial tumors such as carcinomas. Altered expression of cytokeratins can indicate the presence and progression of tumors. Cytokeratin profiles including CK MNF116 positive markers provide insights into tumor type and origin. Additionally they relate to liver disorders like cirrhosis where abnormal keratin organization is observed. These proteins show associations with other structural proteins including vimentin in disease states where cell integrity and signaling are often compromised.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 42:1802-1820 PubMed37046045

2023

Exosomal circSPIRE1 mediates glycosylation of E-cadherin to suppress metastasis of renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guannan Shu,Xuanxuan Lu,Yihui Pan,Junjie Cen,Kangbo Huang,Mi Zhou,Jun Lu,Jiaqi Dong,Hui Han,Wei Chen,Juan Lin,Junhang Luo,Jiaxing Zhang

American journal of stem cells 12:1-11 PubMed36937028

2023

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel activates the AldoA/HIF-1α pathway to promote diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Ruihang Ma,Du Juan,Zhixin Yuan,Jiuhui Sun,Mengjun Wang,Yuxuan Li,Yongli Bao,Hongxu Jin

Scientific reports 10:9955 PubMed32572057

2020

Signaling Inhibitors Accelerate the Conversion of mouse iPS Cells into Cancer Stem Cells in the Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Du,Yanning Xu,Saki Sasada,Aung Ko Ko Oo,Ghmkin Hassan,Hafizah Mahmud,Apriliana Cahya Khayrani,Md Jahangir Alam,Kazuki Kumon,Ryo Uesaki,Said M Afify,Hager M Mansour,Neha Nair,Maram H Zahra,Akimasa Seno,Nobuhiro Okada,Ling Chen,Ting Yan,Masaharu Seno

PloS one 15:e0227634 PubMed31978067

2020

Restoration of Mal overcomes the defects of apoptosis in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Tao Yang,Fei Ma,Hao-Tao Zeng,Miao Zhao,Xian-Hai Zeng,Zhi-Qiang Liu,Ping-Chang Yang

Cell and tissue research 379:181-193 PubMed31768712

2019

CD271 promotes STZ-induced diabetic wound healing and regulates epidermal stem cell survival in the presence of the pTrkA receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Rui Zhang,Xiaohong Li,Yongqian Cao,Kaifeng Huang,Jun Ding,Mengyao Liu,Zhang Feng,Siyuan Yin,Jiaxu Ma,Huayu Zhang,Yibing Wang

Artificial cells, nanomedicine, and biotechnology 47:4165-4171 PubMed31713438

2019

Protective mechanism of GPR30 agonist G1 against ultraviolet B-induced injury in epidermal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqin Zhang,Li Li,Yangchun Xu,Xiaoli Zhao,Fuqiu Li

Nature communications 9:5395 PubMed30568162

2018

Cancer-derived exosomal miR-25-3p promotes pre-metastatic niche formation by inducing vascular permeability and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhicheng Zeng,Yuling Li,Yangjian Pan,Xiaoliang Lan,Fuyao Song,Jingbo Sun,Kun Zhou,Xiaolong Liu,Xiaoli Ren,Feifei Wang,Jinlong Hu,Xiaohui Zhu,Wei Yang,Wenting Liao,Guoxin Li,Yanqing Ding,Li Liang

Cancer research 78:6795-6806 PubMed30389700

2018

Loss of TRIM29 Alters Keratin Distribution to Promote Cell Invasion in Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Teruki Yanagi,Masashi Watanabe,Hiroo Hata,Shinya Kitamura,Keisuke Imafuku,Hiroko Yanagi,Akihiro Homma,Lei Wang,Hidehisa Takahashi,Hiroshi Shimizu,Shigetsugu Hatakeyama

American journal of translational research 8:5569-5579 PubMed28078027

2016

Advanced glycosylation end product promotes forkhead box O1 and inhibits Wnt pathway to suppress capacities of epidermal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhu,Peng Wang,Zhimin Yu,Wei Lai,Yi Cao,Pinbo Huang,Qiaodong Xu,Menglei Yu,Junyao Xu,Zitong Huang,Bing Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin antibody [34BE12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (AB191208)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (AB191208)

Immunocytochemistry - Anti-Cytokeratin antibody [34BE12] (AB191208)

Western blot - Anti-Cytokeratin antibody [34BE12] (AB191208)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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