AB82612

Anti-Cytokeratin antibody [MNF116], prediluted

Name: Anti-Cytokeratin antibody [MNF116], prediluted (ab82612) | Abcam
Brand: Abcam Limited
SKU: AB82612
Price: 475 USD
Availability: InStock

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(1 Publication)

Anti-Cytokeratin antibody [MNF116], prediluted (ab82612) is a mouse monoclonal antibody detecting Cytokeratin in Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.

- Trusted since 2009

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

Paraffin embedded human tonsil tissue stained for Cytokeratin using ab82612 in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

ab82612 staining Cytokeratin in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

Overlay histogram showing A431 cells stained with ab82612 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82612, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MNF116

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Immunogen

Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04264

Specificity

This antibody recognizes the keratin polypeptide of 45, 46, and 56.5 kD. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts, and tracheal epithelium.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Cytokeratin antibody [MNF116], prediluted (ab82612) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Other related products
We have a range of other formats of antibody clone [MNF116] also available for your convenience: ab756, ab82612

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratins also known by the abbreviation 'CK' are a class of intermediate filament proteins providing structural support to epithelial cells. Their mass ranges between 40 kDa to 68 kDa. Cytokeratins are expressed in epithelial tissues including the outer layer of skin and line organs such as the gut and respiratory tract. Commonly referenced with specific subtypes CK MNF116 or CK cocktail antibodies are used for detection in laboratory settings. These proteins help maintain cell integrity and protect from mechanical stress.

Biological function summary

Keratins function as a complex to create a stable cytoskeletal network inside epithelial cells. They interact with proteins to form keratin filaments contributing to the mechanical strength and resilience of cells. Cytokeratins also play a role in intracellular signaling and cellular differentiation. They participate in wound healing processes through modulation of cell proliferation and apoptosis. The CK MNF116 marker is especially noted for its application in identifying and characterizing epithelial proteins in pathology.

Pathways

Cytokeratins are involved in pathways related to cell structure and stress response. Specifically they play key roles in the Epidermal Growth Factor (EGF) signaling pathway and the cell cycle control pathway. These pathways involve proteins like epidermal growth factor receptors (EGFR) and cyclins which regulate cell cycle progression. Cytokeratins influence these pathways by impacting cell adhesion and proliferation essential for maintaining epithelial cell functions.

Cytokeratins have significant roles in cancer diagnostics particularly in epithelial tumors such as carcinomas. Altered expression of cytokeratins can indicate the presence and progression of tumors. Cytokeratin profiles including CK MNF116 positive markers provide insights into tumor type and origin. Additionally they relate to liver disorders like cirrhosis where abnormal keratin organization is observed. These proteins show associations with other structural proteins including vimentin in disease states where cell integrity and signaling are often compromised.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:19848 PubMed31882741

2019

ADAR1 function affects HPV replication and is associated to recurrent human papillomavirus-induced dysplasia in HIV coinfected individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Pujantell,Roger Badia,Iván Galván-Femenía,Edurne Garcia-Vidal,Rafael de Cid,Carmen Alcalde,Antonio Tarrats,Marta Piñol,Francesc Garcia,Ana M Chamorro,Boris Revollo,Sebastian Videla,David Parés,Javier Corral,Cristina Tural,Guillem Sirera,José A Esté,Ester Ballana,Eva Riveira-Muñoz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin antibody [MNF116], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

Flow Cytometry - Anti-Cytokeratin antibody [MNF116], prediluted (AB82612)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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