AB54023

Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37]

Name: Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023) | Abcam
Brand: Abcam Limited
SKU: AB54023
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Mouse Monoclonal GB antibody. Suitable for ICC/IF, FuncS (Neut/Block), IHC-Fr, ELISA and reacts with Human cytomegalovirus samples. Cited in 6 publications.

View Alternative Names

UL55, gB, Envelope glycoprotein B

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (AB54023)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (AB54023)

ab54023, staining Cytomegalovirus Glycoprotein B in monocyte-derived dendritic cells infected with Human cytomegalovirus (VHL/E strain), cultured for 3 hours (left panels) and 24 hours (right panels), by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature. Samples were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse monoclonal IgG was used as the secondary antibody.

Image from Haspot F et al., PLoS One. 2012;7(4):e34795. Epub 2012 Apr 9. Fig 2.; doi:10.1371/journal.pone.0034795; April 9, 2012, PLoS ONE 7(4): e34795.

519 FITC

FITC Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HCMV37

Isotype

IgG2a

Carrier free

Reacts with

Human cytomegalovirus

Applications

FuncS (Neut/Block), ICC/IF, ELISA, IHC-Fr

applications

Specificity

This antibody is specific for Cytomegalovirus glycoprotein B late neutralizing antigen. Reacts with MRC-5 infected cells but not uninfected cells.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human cytomegalovirus": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Participates also in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.

See full target information gB

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

ACS sensors 5:147-153 PubMed31850749

2020

Nanoparticle Reservoirs for Paper-Only Immunosensors.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Alba-Patiño,Cristina Adrover-Jaume,Roberto de la Rica

Oncotarget 8:25989-25999 PubMed27517625

2016

CMV70-3P miRNA contributes to the CMV mediated glioma stemness and represents a target for glioma experimental therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ilya V Ulasov,Natalya V Kaverina,Dhimankrishna Ghosh,Marya A Baryshnikova,Zaira G Kadagidze,Apollon I Karseladze,Anatoly Y Baryshnikov,Charles S Cobbs

Placenta 36:667-73 PubMed25814471

2015

Viperin is induced following toll-like receptor 3 (TLR3) ligation and has a virus-responsive function in human trophoblast cells.

Applications

Unspecified application

Species

Unspecified reactive species

B Wang,Y Fang,Y Wu,K Koga,Y Osuga,S Lv,D Chen,Y Zhu,J Wang,H Huang

Clinical and vaccine immunology : CVI 21:174-80 PubMed24334684

2013

Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity.

Applications

Neut

Species

Unspecified reactive species

Marc Kirchmeier,Anne-Catherine Fluckiger,Catalina Soare,Jasminka Bozic,Barthelemy Ontsouka,Tanvir Ahmed,Abebaw Diress,Lenore Pereira,Florian Schödel,Stanley Plotkin,Charlotte Dalba,David Klatzmann,David E Anderson

PloS one 7:e34795 PubMed22496863

2012

Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner.

Applications

ICC/IF

Species

Unspecified reactive species

Fabienne Haspot,Amélie Lavault,Christian Sinzger,Kerstin Laib Sampaio,York-Dieter Stierhof,Paul Pilet,Céline Bressolette-Bodin,Franck Halary

The EMBO journal 5:3057-63 PubMed3024973

1986

Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus.

Applications

Unspecified application

Species

Unspecified reactive species

M P Cranage,T Kouzarides,A T Bankier,S Satchwell,K Weston,P Tomlinson,B Barrell,H Hart,S E Bell,A C Minson

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37]

Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (AB54023)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise