AB6502

Anti-Cytomegalovirus pp28 antibody [5C3]

Name: Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502) | Abcam
Brand: Abcam Limited
SKU: AB6502

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(12 Publications)

Mouse Monoclonal Cytomegalovirus pp28 antibody. Carrier free. Suitable for WB, ELISA, ICC/IF and reacts with Human cytomegalovirus samples. Cited in 12 publications.

View Alternative Names

28 kDa structural phosphoprotein, CMV pp28 tegument protein, CMV tegument protein UL99, Cytomegalovirus pp28 tegument protein, Cytomegalovirus tegument protein UL99, HCMV, HHV 5, Human Herpesvirus 5, Tegument protein UL99, UL 99

2 Images

ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (AB6502)

ELISA

Unknown

ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (AB6502)

Please see protocols tab for procedure. Antigen is CMV infected cell extract coated at a dilution of 1/100.

Unknown

Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (AB6502)

See protocols tab for procedure. Antigen is CMV infected cell extract at 10 μg/cm.

All lanes:

Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

Predicted band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human cytomegalovirus

Applications

ICC/IF, ELISA, WB

applications

Specificity

Cytomegalovirus pp28 tegument protein. Slight cross reactivity with HSV1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human cytomegalovirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/16000", "WB-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/102400", "ELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

> 95% IgG by SDS-PAGE.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Cytomegalovirus pp28

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36232315

2022

The Human Cytomegalovirus β2.7 Long Non-Coding RNA Prevents Induction of Reactive Oxygen Species to Maintain Viral Gene Silencing during Latency.

Applications

Unspecified application

Species

Unspecified reactive species

Marianne R Perera,John H Sinclair

Viruses 13: PubMed34065234

2021

: An Screening Targeting HCMV ppUL44 Processivity Factor Homodimerization Identifies Small Molecules Inhibiting Viral Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Hanieh Ghassabian,Federico Falchi,Martina Timmoneri,Beatrice Mercorelli,Arianna Loregian,Giorgio Palù,Gualtiero Alvisi

Journal of virology 90:9664-9673 PubMed27535048

2016

Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1.

Applications

Species

Unspecified reactive species

Yongxu Lu,Anne Orr,Roger D Everett

Journal of virology 90:8621-33 PubMed27440897

2016

MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Sloan,Anne Orr,Roger D Everett

Journal of virology 89:3062-75 PubMed25552717

2015

Analysis of the functional interchange between the IE1 and pp71 proteins of human cytomegalovirus and ICP0 of herpes simplex virus 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxu Lu,Roger D Everett

Genes & development 27:1809-20 PubMed23964095

2013

A new role for the cellular PABP repressor Paip2 as an innate restriction factor capable of limiting productive cytomegalovirus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Caleb McKinney,Dong Yu,Ian Mohr

Journal of virology 86:4091-101 PubMed22345456

2012

The human cytomegalovirus-specific UL1 gene encodes a late-phase glycoprotein incorporated in the virion envelope.

Applications

ICC/IF

Species

Human

Medya Shikhagaie,Eva Mercé-Maldonado,Elena Isern,Aura Muntasell,M Mar Albà,Miguel López-Botet,Hartmut Hengel,Ana Angulo

Immunology and cell biology 89:753-66 PubMed21670740

2011

Human cytomegalovirus UL7, a homologue of the SLAM-family receptor CD229, impairs cytokine production.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Engel,Natàlia Pérez-Carmona,M Mar Albà,Kevin Robertson,Peter Ghazal,Ana Angulo

The Journal of clinical investigation 120:3191-208 PubMed20679731

2010

Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.

Applications

ICC/IF

Species

Unspecified reactive species

Richard J Stanton,Katarina Baluchova,Derrick J Dargan,Charles Cunningham,Orla Sheehy,Sepehr Seirafian,Brian P McSharry,M Lynne Neale,James A Davies,Peter Tomasec,Andrew J Davison,Gavin W G Wilkinson

Journal of virology 83:11421-8 PubMed19741001

2009

The endoplasmic reticulum chaperone BiP/GRP78 is important in the structure and function of the human cytomegalovirus assembly compartment.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Buchkovich,Tobi G Maguire,Adrienne W Paton,James C Paton,James C Alwine

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytomegalovirus pp28 antibody [5C3]

ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (AB6502)

Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (AB6502)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

Product promise