Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1]

• Flow Cyt

Lab

Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).

• IP

Lab

Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905)

Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 μg with ab23905 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

Lane 1 : U-87 MG whole cell lysate 10 μg

Lane 2 : ab23905 IP in U-87 MG whole cell lysate

Lane 3 : Mouse monoclonal IgG instead of ab23905 in U-87 MG whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second

All lanes:

Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

Predicted band size: 73 kDa

false

Exposure time: 1s

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905)

Flow Cytometry analysis of C6 (rat glial tumor cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).

• IP

Lab

Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905)

Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 μg with ab23905 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

Lane 1 : Rat brain tissue lysate 10 μg

Lane 2 : ab23905 IP in Rat brain tissue lysate

Lane 3 : Mouse monoclonal IgG instead of ab23905 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second

All lanes:

Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

Predicted band size: 73 kDa

false

• WB

Lab

Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905)

Blocking buffer and concentration : 5% NFDM/TBST

Exposure time :

Lanes 1-3 : 37 seconds
Lanes 4-6 : 81 seconds

All lanes:

Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

• WB

AbReview18030****

Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905)

Gel : 10% acryl amid
Blocking Step : 5% Milk for 30 minutes at 25°C

This image was generated from the hybridoma version of the product.

All lanes:

Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution

All lanes:

Rat Sciatic nerve whole tissue lysate at 60 µg

Secondary

All lanes:

HRP-conjugated Goat anti-mouse IgG at 1/10000 dilution

Predicted band size: 73 kDa

true

This image is courtesy of an anonymous Abreview