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Mouse Recombinant Monoclonal DC1I1 antibody. Suitable for IP, Flow Cyt, WB and reacts with Rat, Human, Mouse samples. Cited in 41 publications.


Images

Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905), expandable thumbnail
  • Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905), expandable thumbnail
  • Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905), expandable thumbnail
  • Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905), expandable thumbnail
  • Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (AB23905), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC
Human
Tested
Tested
Tested
Not recommended
Mouse
Expected
Expected
Tested
Not recommended
Rat
Tested
Tested
Tested
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.

Alternative names

DNCI1, DNCIC1, DYNC1I1, Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, DH IC-1

Recommended products

Mouse Recombinant Monoclonal DC1I1 antibody. Suitable for IP, Flow Cyt, WB and reacts with Rat, Human, Mouse samples. Cited in 41 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
74.1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 8th March 2021.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905), expandable thumbnail

    Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).

  • Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905), expandable thumbnail

    Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Flow Cytometry analysis of C6 (rat glial tumor cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).

  • Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Gel: 10% acryl amid
    Blocking Step: 5% Milk for 30 minutes at 25°C

    This image was generated from the hybridoma version of the product.

    All lanes: Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution

    All lanes: Rat Sciatic nerve whole tissue lysate at 60 µg

    Secondary

    All lanes: HRP-conjugated Goat anti-mouse IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 73 kDa

  • Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905), expandable thumbnail

    Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 μg with ab23905 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/10000 dilution.

    Lane 1: Rat brain tissue lysate 10 μg

    Lane 2: ab23905 IP in Rat brain tissue lysate

    Lane 3: Mouse monoclonal IgG instead of ab23905 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    All lanes: Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Predicted band size: 73 kDa

  • Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905), expandable thumbnail

    Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 μg with ab23905 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/10000 dilution.

    Lane 1: U-87 MG whole cell lysate 10 μg

    Lane 2: ab23905 IP in U-87 MG whole cell lysate

    Lane 3: Mouse monoclonal IgG instead of ab23905 in U-87 MG whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    All lanes: Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Predicted band size: 73 kDa

    Exposure time: 1s

  • Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905), expandable thumbnail

    Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905)

    Blocking buffer and concentration: 5% NFDM/TBST

    Exposure time:

    Lanes 1-3: 37 seconds
    Lanes 4-6: 81 seconds

    All lanes: Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Lane 5: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Lane 6: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 73 kDa

    Observed band size: 73 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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