AB104252

Anti-Cytosolic Phospholipase A2 antibody

Name: Anti-Cytosolic Phospholipase A2 antibody (ab104252) | Abcam
Brand: Abcam Limited
SKU: AB104252
Price: 535 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal Cytosolic Phospholipase A2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PLA2G4A aa 150-200.

View Alternative Names

CPLA2, PLA2G4, PLA2G4A, Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA

2 Images

Unknown

Western blot - Anti-Cytosolic Phospholipase A2 antibody (AB104252)

All lanes:

Western blot - Anti-Cytosolic Phospholipase A2 antibody (ab104252) at 1 µg

All lanes:

HeLa cell lysate at 35 µg

Predicted band size: 85 kDa

Observed band size: 90 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody (AB104252)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody (AB104252)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation with ab104252 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml) (green). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PLA2G4A aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P47712

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab104252 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed : 10358058, PubMed : 14709560, PubMed : 16617059, PubMed : 17472963, PubMed : 18451993, PubMed : 27642067, PubMed : 7794891, PubMed : 8619991, PubMed : 8702602, PubMed : 9425121). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 8619991, PubMed : 9425121). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 9425121). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed : 27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed : 7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed : 12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed : 7794891).

See full target information PLA2G4A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:761 PubMed38909140

2024

Hog1 acts in a Mec1-independent manner to counteract oxidative stress following telomerase inactivation in Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Bechara Zeinoun,Maria Teresa Teixeira,Aurélia Barascu

International journal of molecular sciences 20: PubMed31569627

2019

Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Maja Tunset,Astrid Jullumstrø Feuerherm,Linn-Karina Myrland Selvik,Berit Johansen,Siver Andreas Moestue

International journal of clinical and experimental pathology 10:7873-7881 PubMed31966635

2017

Overexpression of Sox3 is associated with promoted tumor progression and poor prognosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Feng,Feng Xiao,Ning Yang,Nan Zhu,Yong Fu,Hai-Bin Zhang,Guang-Shun Yang

View all publications

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Product promise

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Anti-Cytosolic Phospholipase A2 antibody

Western blot - Anti-Cytosolic Phospholipase A2 antibody (AB104252)

Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody (AB104252)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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