AB239730

Anti-Cytosolic Phospholipase A2 antibody [C1]

Name: Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) | Abcam
Brand: Abcam Limited
SKU: AB239730
Price: 500 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal Cytosolic Phospholipase A2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2G4A aa 550 to C-terminus.

View Alternative Names

CPLA2, PLA2G4, PLA2G4A, Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Paraffin-embedded human pancreas tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Paraffin-embedded human stomach tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Paraffin-embedded human kidney tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Paraffin-embedded human glioma tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

All lanes:

Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 1 µg/mL

Lane 1:

Human lung lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

All lanes:

Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 5 µg/mL

All lanes:

Recombinant human Cytosolic Phospholipase A2 protein

Predicted band size: 85 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PLA2G4A aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P47712

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed : 10358058, PubMed : 14709560, PubMed : 16617059, PubMed : 17472963, PubMed : 18451993, PubMed : 27642067, PubMed : 7794891, PubMed : 8619991, PubMed : 8702602, PubMed : 9425121). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 8619991, PubMed : 9425121). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 9425121). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed : 27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed : 7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed : 12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed : 7794891).

See full target information PLA2G4A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iMeta 4:e70007 PubMed40236767

2025

Gut microbiota-derived tryptophan metabolites improve total parenteral nutrition-associated infections by regulating Group 3 innate lymphoid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Longchang Huang,Peng Wang,Shuai Liu,Guifang Deng,Xin Qi,Guangming Sun,Xuejin Gao,Li Zhang,Yupeng Zhang,Yaqin Xiao,Tingting Gao,Gulisudumu Maitiabula,Xinying Wang

International journal of molecular sciences 24: PubMed36674425

2023

Protective Effects of Human Pericyte-like Adipose-Derived Mesenchymal Stem Cells on Human Retinal Endothelial Cells in an In Vitro Model of Diabetic Retinopathy: Evidence for Autologous Cell Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella Lupo,Aleksandra Agafonova,Alessia Cosentino,Giovanni Giurdanella,Giuliana Mannino,Debora Lo Furno,Ivana Roberta Romano,Rosario Giuffrida,Floriana D'Angeli,Carmelina Daniela Anfuso

Journal of nanobiotechnology 19:417 PubMed34903236

2021

A novel biomimetic nanomedicine system with anti-inflammatory and anti-osteoporosis effects improves the therapy efficacy of steroid-resistant nephrotic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Mingyi Zhao,Xinying Xiang,Qingnan He,Rong Gui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytosolic Phospholipase A2 antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Western blot - Anti-Cytosolic Phospholipase A2 antibody [C1] (AB239730)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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