AB53105

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody

Name: Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105) | Abcam
Brand: Abcam Limited
SKU: AB53105
Price: 525 USD
Availability: InStock

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(15 Publications)

Rabbit Polyclonal Cytosolic Phospholipase A2 phospho S505 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PLA2G4A phospho S505.

View Alternative Names

CPLA2, PLA2G4, PLA2G4A, Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

ab53105 at 1/50 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

ICC/IF image of ab53105 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53105, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

All lanes:

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105) at 1/300 dilution

Lane 1:

extracts from HeLa cells treated with TNF-a (20ng/ml, 30mins)

Lane 2:

extracts from HeLa cells treated with TNF-a (20ng/ml, 30mins) with immunising peptide

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PLA2G4A phospho S505. The exact immunogen used to generate this antibody is proprietary information.

P47712

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab53105 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed : 10358058, PubMed : 14709560, PubMed : 16617059, PubMed : 17472963, PubMed : 18451993, PubMed : 27642067, PubMed : 7794891, PubMed : 8619991, PubMed : 8702602, PubMed : 9425121). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 8619991, PubMed : 9425121). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 9425121). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed : 27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed : 7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed : 12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed : 7794891).

See full target information PLA2G4A phospho S505

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical biology 59:672-682 PubMed34078224

2021

Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbin Sun,Sixing Huang,Yao Qin,Ping Zhang,Ziwei Li,Li Zhang,Xin Wang,Ruijun Wu,Shaorong Qin,Jiayong Huo,Kunquan Xiao,Weizao Luo

Scientific reports 10:22265 PubMed33335297

2020

Gigantol ameliorates CCl-induced liver injury via preventing activation of JNK/cPLA2/12-LOX inflammatory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Xue,Qiangqiang Deng,Qingli Zhang,Zhenghua Ma,Binfan Chen,Xiaolu Yu,Huige Peng,Sheng Yao,Jia Liu,Yang Ye,Guoyu Pan

FASEB journal : official publication of the Federa 33:14281-14295 PubMed31661303

2019

Cytosolic phospholipase A2 is a key regulator of blood-brain barrier function in epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Anika M S Hartz,Ralf G Rempe,Emma L B Soldner,Anton Pekcec,Juli Schlichtiger,Richard Kryscio,Bjoern Bauer

International journal of molecular sciences 20: PubMed31569627

2019

Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Maja Tunset,Astrid Jullumstrø Feuerherm,Linn-Karina Myrland Selvik,Berit Johansen,Siver Andreas Moestue

Journal of cellular physiology 234:9592-9604 PubMed30317637

2018

Brain-derived neurotrophic factor alleviates the oxidative stress induced by oxygen and glucose deprivation in an ex vivo brain slice model.

Applications

Unspecified application

Species

Unspecified reactive species

Paloma González-Rodríguez,Irene F Ugidos,Diego Pérez-Rodríguez,Berta Anuncibay-Soto,María Santos-Galdiano,Enrique Font-Belmonte,José Manuel Gonzalo-Orden,Arsenio Fernández-López

Biochimica et biophysica acta. Molecular basis of 1864:2957-2971 PubMed29894755

2018

Activation of bradykinin B2 receptor induced the inflammatory responses of cytosolic phospholipase A after the early traumatic brain injury.

Applications

Species

Unspecified reactive species

Honglu Chao,Yinlong Liu,Chao Lin,Xiupeng Xu,Zheng Li,Zhongyuan Bao,Liang Fan,Chao Tao,Lin Zhao,Yan Liu,Xiaoming Wang,Yongping You,Ning Liu,Jing Ji

Cancer research 76:4608-18 PubMed27335109

2016

Myc Expression Drives Aberrant Lipid Metabolism in Lung Cancer.

Applications

IHC-P

Species

Human

Zoe Hall,Zsuzsanna Ament,Catherine H Wilson,Deborah L Burkhart,Tom Ashmore,Albert Koulman,Trevor Littlewood,Gerard I Evan,Julian L Griffin

Scientific reports 6:26076 PubMed27188343

2016

Metabolomics Investigation Reveals Metabolite Mediators Associated with Acute Lung Injury and Repair in a Murine Model of Influenza Pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Cui,Dahai Zheng,Yie Hou Lee,Tze Khee Chan,Yadunanda Kumar,Wanxing Eugene Ho,Jian Zhu Chen,Steven R Tannenbaum,Choon Nam Ong

Molecular pain 9:29 PubMed23773289

2013

An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model.

Applications

IHC-Fr

Species

Mouse

Lin Ma,Jun Nagai,Jerold Chun,Hiroshi Ueda

Blood 121:4586-94 PubMed23620575

2013

LIM kinase-1 selectively promotes glycoprotein Ib-IX-mediated TXA2 synthesis, platelet activation, and thrombosis.

Applications

Unspecified application

Species

Mouse

Brian Estevez,Aleksandra Stojanovic-Terpo,M Keegan Delaney,Kelly A O'Brien,Michael C Berndt,Changgeng Ruan,Xiaoping Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cytosolic Phospholipase A2 (phospho S505) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (AB53105)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (15)

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