AB191605

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cytosolic Phospholipase A2 phospho S505 antibody. Suitable for IP, Dot, WB and reacts with Mouse, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

CPLA2, PLA2G4, PLA2G4A, Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA

3 Images

Lab

Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (AB191605)

ab191605 at 1/50 dilution immunoprecipitating Cytosolic Phospholipase A2 (phospho S505) in NIH/3T3 (mouse embryo) whole cell lysate.

Lane 1 (input) : NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate 10μg

Lane 2 (+) : ab191605 + NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab191605 in NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate

For western blotting, ab191605 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (AB191605)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605) at 1/2000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 20 ng/ml TNF-alpha for 10 minutes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 10s

Supplier Data

Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (AB191605)

Dot blot analysis of Cytosolic Phospholipase A2 (phospho S505) phospho peptide (Lane 1) and Phospholipase A2 Non-phospho peptide (Lane 2) labeled using ab191605 at 1/1000 dilution followed by Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18552

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Dot, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed : 10358058, PubMed : 14709560, PubMed : 16617059, PubMed : 17472963, PubMed : 18451993, PubMed : 27642067, PubMed : 7794891, PubMed : 8619991, PubMed : 8702602, PubMed : 9425121). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 8619991, PubMed : 9425121). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 9425121). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed : 27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed : 7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed : 12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed : 7794891).

See full target information PLA2G4A phospho S505

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2000 PubMed40011517

2025

Dual-function regulator MexL as a target to control phenazines production and pathogenesis of Pseudomonas aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoxiao Yu,Zhikun Wu,Dejian Liu,Haoyu Liu,Yu Zhang,Yaqian Zheng,Yanhong Huang,Shumin Liao,Yu Wei,Wei Huang,Zhenyu Zhang,Xi Liu,Haiying Yu,Di Wang,Liang Li,Feng Long,Luyan Z Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552]

Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (AB191605)

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (AB191605)

Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (AB191605)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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