AB251094

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Cytosolic Phospholipase A2 phospho S505 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Mouse, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

CPLA2, PLA2G4, PLA2G4A, Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA

3 Images

Supplier Data

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (AB251094)

This data was developed using ab191605, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (<a href='/en-us/products/primary-antibodies/cytosolic-phospholipase-a2-phospho-s505-antibody-epr18552-ab191605'>ab191605</a>) at 1/2000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 20 ng/ml TNF-alpha for 10 minutes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 10s

Lab

Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (AB251094)

This data was developed using ab191605, the same antibody clone in a different buffer formulation.

ab191605 at 1/50 dilution immunoprecipitating Cytosolic Phospholipase A2 (phospho S505) in NIH/3T3 (mouse embryo) whole cell lysate.

Lane 1 (input) : NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate 10μg

Lane 2 (+) : ab191605 + NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab191605 in NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate

For western blotting, ab191605 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (<a href='/en-us/products/primary-antibodies/cytosolic-phospholipase-a2-phospho-s505-antibody-epr18552-ab191605'>ab191605</a>)

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 1s

Supplier Data

Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (AB251094)

This data was developed using ab191605, the same antibody clone in a different buffer formulation. Dot blot analysis of Cytosolic Phospholipase A2 (phospho S505) phospho peptide (Lane 1) and Phospholipase A2 Non-phospho peptide (Lane 2) labeled using ab191605 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18552

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251094 is the carrier-free version of ab191605.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed : 10358058, PubMed : 14709560, PubMed : 16617059, PubMed : 17472963, PubMed : 18451993, PubMed : 27642067, PubMed : 7794891, PubMed : 8619991, PubMed : 8702602, PubMed : 9425121). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 8619991, PubMed : 9425121). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed : 10358058, PubMed : 17472963, PubMed : 18451993, PubMed : 7794891, PubMed : 9425121). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed : 27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed : 7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed : 12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed : 7794891).

See full target information PLA2G4A phospho S505

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free

Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (AB251094)

Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (AB251094)

Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (AB251094)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Product promise