Rabbit Recombinant Monoclonal D Box Binding Protein antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate samples.
Images
![Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--western-blot-img15568.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112)")
![Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--immunoprecipitation-img3780.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112)")
![Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--western-blot-img37094.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112)")
![Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--flow-cytometry-intracellular-img105215.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112)")
![Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--immunocytochemistry-immunofluorescence-img98571.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (AB222112)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/600 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
Alternative names
D site-binding protein, Albumin D box-binding protein, Albumin D-element-binding protein, Tax-responsive enhancer element-binding protein 302, TaxREB302, DBP
Recommended products
Rabbit Recombinant Monoclonal D Box Binding Protein antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22370-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--western-blot-img15568.jpg "Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)")
Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The DBP protein is ubiquitously expressed but mainly at suprachiasmatic nuclei in the hypothalamus or liver, and fine-tuned by circadian clock. The antibody detects a band around the reported molecular weight of DBP in human hypothalamus and brain tissue lysates. Negative control: testis (PMID: 2331750)
Exposure time: 3 minutes.
All lanes: Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Human liver lysate at 20 µg
Lane 5: Human hypothalamus lysate at 20 µg
Lane 6: Human brain lysate at 20 µg
Lane 7: Human testis lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 45 kDa
![Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--immunoprecipitation-img3780.jpg "Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)")
Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
D Box Binding Protein was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector, whole cell lysate, with ab222112 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab222112 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate 10ug.
Lane 2: ab222112 IP in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab222112 in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
All lanes: Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
Predicted band size: 34 kDa
![Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--western-blot-img37094.jpg "Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)")
Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
All lanes: Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: HEK-293T transfected with D Box Binding Protein (WT) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 52 kDa
![Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--flow-cytometry-intracellular-img105215.jpg "Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)")
Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-his tagged D Box Binding Protein expression vector) cells labelling D Box Binding Protein with ab222112 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Secondary antibody isGoat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution.
Cells were stained with rabbit IgG (Left) or ab222112 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.
![Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-ab222112--immunocytochemistry-immunofluorescence-img98571.jpg "Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)")
Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling D Box Binding Protein with ab222112 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged D Box Binding Protein expression vector. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com