Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling D Box Binding Protein with ab222112 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged D Box Binding Protein expression vector. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222112).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-his tagged D Box Binding Protein expression vector) cells labelling D Box Binding Protein with ab222112 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Secondary antibody is Goat anti rabbit IgG (Alexa Fluor^® 647, ab150079) at 1/2000 dilution.

Cells were stained with rabbit IgG (Left) or ab222112 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor^® 488.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222112).

• IP

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Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022)

D Box Binding Protein was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector, whole cell lysate, with ab222112 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab222112 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate 10ug.

Lane 2 : ab222112 IP in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab222112 in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222112).

All lanes:

Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (<a href='/en-us/products/primary-antibodies/d-box-binding-protein-antibody-epr22370-51-ab222112'>ab222112</a>)

Predicted band size: 34 kDa

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