Rabbit Recombinant Monoclonal D Box Binding Protein antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-bsa-and-azide-free-ab263022--immunoprecipitation-img3780.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022)")
![Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-bsa-and-azide-free-ab263022--flow-cytometry-intracellular-img105215.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022)")
![Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-bsa-and-azide-free-ab263022--immunocytochemistry-immunofluorescence-img98571.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (AB263022)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
Alternative names
D site-binding protein, Albumin D box-binding protein, Albumin D-element-binding protein, Tax-responsive enhancer element-binding protein 302, TaxREB302, DBP
Recommended products
Rabbit Recombinant Monoclonal D Box Binding Protein antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22370-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab263022 is the carrier-free version of Anti-D Box Binding Protein antibody [EPR22370-51] ab222112.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-bsa-and-azide-free-ab263022--immunoprecipitation-img3780.jpg "Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)")
Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
D Box Binding Protein was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector, whole cell lysate, with Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate 10ug.
Lane 2: Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 IP in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-D Box Binding Protein antibody [EPR22370-51] ab222112).
All lanes: Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (Anti-D Box Binding Protein antibody [EPR22370-51] ab222112)
Predicted band size: 34 kDa
![Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-bsa-and-azide-free-ab263022--flow-cytometry-intracellular-img105215.jpg "Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)")
Flow Cytometry (Intracellular) - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-his tagged D Box Binding Protein expression vector) cells labelling D Box Binding Protein with Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Secondary antibody is Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution.
Cells were stained with rabbit IgG (Left) or Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-D Box Binding Protein antibody [EPR22370-51] ab222112).
![Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022), expandable thumbnail](https://content.abcam.com/products/images/d-box-binding-protein-antibody-epr22370-51-bsa-and-azide-free-ab263022--immunocytochemistry-immunofluorescence-img98571.jpg "Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)")
Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling D Box Binding Protein with Anti-D Box Binding Protein antibody [EPR22370-51] ab222112 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged D Box Binding Protein expression vector. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-D Box Binding Protein antibody [EPR22370-51] ab222112).
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