AB233516

Anti-D2HGDH antibody

Name: Anti-D2HGDH antibody (ab233516) | Abcam
Brand: Abcam Limited
SKU: AB233516
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal D2HGDH antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human D2HGDH aa 50-250.

View Alternative Names

D2HGD, D2HGDH

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human glioma tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human testis tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Supplier Data

Western blot - Anti-D2HGDH antibody (AB233516)

All lanes:

Western blot - Anti-D2HGDH antibody (ab233516) at 1 µg/mL

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 56 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human liver tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Supplier Data

Western blot - Anti-D2HGDH antibody (AB233516)

All lanes:

Western blot - Anti-D2HGDH antibody (ab233516) at 1 µg/mL

All lanes:

Rat kidney tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-D2HGDH antibody (AB233516)

All lanes:

Western blot - Anti-D2HGDH antibody (ab233516) at 2 µg/mL

All lanes:

Recombinant human D2HGDH protein

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-D2HGDH antibody (AB233516)

All lanes:

Western blot - Anti-D2HGDH antibody (ab233516) at 1 µg/mL

All lanes:

Rat liver tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human D2HGDH aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q8N465

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate (PubMed : 15070399, PubMed : 15609246, PubMed : 16037974, PubMed : 20020533, PubMed : 33431826). Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC) (PubMed : 33431826). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC (PubMed : 33431826).

See full target information D2HGDH

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and function 43:e70116 PubMed40864431

2025

The Extracellular Matrix Agrin Promotes Epicardial Cells Activation and Proliferation Post Myocardial Infarction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Han,Dinghui Wang,Huiping Yang,Xiaodong Jing,Qiang She,Bin Liu

Cell reports 33:108264 PubMed33053349

2020

Activation of the CARD8 Inflammasome Requires a Disordered Region.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley J Chui,Andrew R Griswold,Cornelius Y Taabazuing,Elizabeth L Orth,Kuo Gai,Sahana D Rao,Daniel P Ball,Jeffrey C Hsiao,Daniel A Bachovchin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-D2HGDH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Western blot - Anti-D2HGDH antibody (AB233516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (AB233516)

Western blot - Anti-D2HGDH antibody (AB233516)

Western blot - Anti-D2HGDH antibody (AB233516)

Western blot - Anti-D2HGDH antibody (AB233516)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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Proteins & Peptides

Primary Antibodies

Primary Antibodies

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