Rabbit Recombinant Monoclonal D4 GDI antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples.
Images
![Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (AB181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--immunoprecipitation-img46496.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (AB181259)")
![Western blot - Anti-D4 GDI antibody [EPR14211(B)] (AB181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--western-blot-img94985.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-D4 GDI antibody [EPR14211(B)] (AB181259)")
![Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (AB181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--immunocytochemistry-immunofluorescence-img43798.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (AB181259)")
![Western blot - Anti-D4 GDI antibody [EPR14211(B)] (AB181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--western-blot-img126025.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-D4 GDI antibody [EPR14211(B)] (AB181259)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 - 1/60 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:7512369, PubMed:8356058). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Alternative names
GDIA2, GDID4, RAP1GN1, ARHGDIB, Rho GDP-dissociation inhibitor 2, Rho GDI 2, Ly-GDI, Rho-GDI beta
Recommended products
Rabbit Recombinant Monoclonal D4 GDI antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14211(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (ab181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--immunoprecipitation-img46496.jpg "Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)")
Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181259 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
Predicted band size: 22 kDa
![Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--western-blot-img94985.jpg "Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)")
Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
All lanes: Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/10000 dilution
All lanes: Human bone marrow lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Predicted band size: 22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (ab181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--immunocytochemistry-immunofluorescence-img43798.jpg "Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)")
Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
Immunofluorescent analysis of HL60 cells (-20°Acetone-fixed) labeling D4 GDI with ab181259 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] (ab181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139051.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling D4 GDI with ab181259 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259), expandable thumbnail](https://content.abcam.com/products/images/d4-gdi-antibody-epr14211b-ab181259--western-blot-img126025.jpg "Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)")
Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259)
All lanes: Western blot - Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/50000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: U937 cell lysate at 20 µg
Lane 3: HL60 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com