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AB307160

Anti-DAAM1 antibody [EPR25376-223]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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Rabbit Recombinant Monoclonal DAAM1 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples.

View Alternative Names

KIAA0666, DAAM1, Disheveled-associated activator of morphogenesis 1

9 Images
Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on human breast carcinoma (PMID : 22655072). The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat cardiac muscle. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat testis. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat cerebrum. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Buffer/dilution : 5% NFDM/TBST Exposure time : 180 seconds Negative control : NCI-H460 (PMID : 20927366) The identity of the bands below 75 kDa are unknown.

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (ab307160) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

HT-29 whole cell lysate at 20 µg

Lane 3:

U-2 OS whole cell lysate at 20 µg

Lane 4:

NCI-H460 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa

false

Exposure time: 180s

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Buffer/dilution concentration : 5% NFDM/TBST.

This antibody does not cross-react with human DAAM2.

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (ab307160) at 1/1000 dilution

Lane 1:

His-tagged human DAAM1 fragment at 10 ng

Lane 2:

His-tagged human DAAM2 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,60 kDa

false

Exposure time: 15s

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Buffer/dilution concentration : 5% NFDM/TBST

The identity of the bands below 75 kDa are unknown.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (ab307160) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

SH-SY5Y whole cell lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa

true

Exposure time: 180s

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)
  • WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] (AB307160)

Buffer/dilution concentration : 5% NFDM/TBST Exposure : Lanes 1,3 : 180 seconds, Lanes 2,4, and 5 : 92 seconds

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (ab307160) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Neuro-2a whole cell lysate at 20 µg

Lane 4:

C2C12 whole cell lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25376-223

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed : 16630611, PubMed : 17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity). During cell division, may regulate RHOA activation that signals spindle orientation and chromosomal segregation.
See full target information DAAM1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com