Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)

Rabbit Monoclonal DAAM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse, Recombinant fragment - Human samples.

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Images

Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-P	WB
Human	Not recommended	Not recommended	Not recommended	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Tested
Rat	Not recommended	Not recommended	Not recommended	Tested	Tested
Recombinant fragment - Human	Not recommended	Not recommended	Not recommended	Not recommended	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information DAAM1_HUMAN

Function

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity). During cell division, may regulate RHOA activation that signals spindle orientation and chromosomal segregation.

Alternative names

KIAA0666, DAAM1, Disheveled-associated activator of morphogenesis 1

Recommended products

Rabbit Monoclonal DAAM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25376-223
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Buffer/dilution concentration: 5% NFDM/TBST.
The identity of the bands below 75 kDa are unknown.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-DAAM1 antibody [EPR25376-223] (Anti-DAAM1 antibody [EPR25376-223] ab307160) at 1/1000 dilution
Lane 1: Human lung tissue lysate at 20 µg
Lane 2: SH-SY5Y whole cell lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 123 kDa
Exposure time: 180s
Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Buffer/dilution concentration: 5% NFDM/TBST

Exposure: Lanes 1,3: 180 seconds, Lanes 2,4, and 5: 92 seconds
All lanes: Western blot - Anti-DAAM1 antibody [EPR25376-223] (Anti-DAAM1 antibody [EPR25376-223] ab307160) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Neuro-2a whole cell lysate at 20 µg
Lane 4: C2C12 whole cell lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 123 kDa
Exposure time: 180s
Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Buffer/dilution: 5% NFDM/TBST

Exposure time: 180 seconds

Negative control: NCI-H460 (PMID: 20927366)

The identity of the bands below 75 kDa are unknown.
All lanes: Western blot - Anti-DAAM1 antibody [EPR25376-223] (Anti-DAAM1 antibody [EPR25376-223] ab307160) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: HT-29 whole cell lysate at 20 µg
Lane 3: U-2 OS whole cell lysate at 20 µg
Lane 4: NCI-H460 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 123 kDa
Exposure time: 180s
Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Buffer/dilution concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.

This antibody does not cross-react with human DAAM2.
All lanes: Western blot - Anti-DAAM1 antibody [EPR25376-223] (Anti-DAAM1 antibody [EPR25376-223] ab307160) at 1/1000 dilution
Lane 1: His-tagged human DAAM1 fragment at 10 ng
Lane 2: His-tagged human DAAM2 fragment at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30 kDa, 60 kDa
Exposure time: 15s
Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DAAM1 with Anti-DAAM1 antibody [EPR25376-223] ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on rat cerebrum.

The section was incubated with Anti-DAAM1 antibody [EPR25376-223] ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DAAM1 with Anti-DAAM1 antibody [EPR25376-223] ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on rat testis.

The section was incubated with Anti-DAAM1 antibody [EPR25376-223] ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling DAAM1 with Anti-DAAM1 antibody [EPR25376-223] ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on rat cardiac muscle.

The section was incubated with Anti-DAAM1 antibody [EPR25376-223] ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling DAAM1 with Anti-DAAM1 antibody [EPR25376-223] ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on human cardiac muscle.

The section was incubated with Anti-DAAM1 antibody [EPR25376-223] ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (ab307161)
This data was developed using Anti-DAAM1 antibody [EPR25376-223] ab307160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling DAAM1 with Anti-DAAM1 antibody [EPR25376-223] ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on human breast carcinoma (PMID: 22655072).

The section was incubated with Anti-DAAM1 antibody [EPR25376-223] ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.