Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on human breast carcinoma (PMID : 22655072). The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat cardiac muscle. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat testis. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DAAM1 with ab307160 at 1/2000 dilution (0.263 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat cerebrum. The section was incubated with ab307160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Buffer/dilution concentration : 5% NFDM/TBST. Exposure time : 15 seconds. This antibody does not cross-react with human DAAM2.

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (<a href='/en-us/products/primary-antibodies/daam1-antibody-epr25376-223-ab307160'>ab307160</a>) at 1/1000 dilution

Lane 1:

His-tagged human DAAM1 fragment at 10 ng

Lane 2:

His-tagged human DAAM2 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,60 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation.

Buffer/dilution concentration : 5% NFDM/TBST.

The identity of the bands below 75 kDa are unknown.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (<a href='/en-us/products/primary-antibodies/daam1-antibody-epr25376-223-ab307160'>ab307160</a>) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

SH-SY5Y whole cell lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Buffer/dilution : 5% NFDM/TBST Exposure time : 180 seconds Negative control : NCI-H460 (PMID : 20927366) The identity of the bands below 75 kDa are unknown.

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (<a href='/en-us/products/primary-antibodies/daam1-antibody-epr25376-223-ab307160'>ab307160</a>) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

HT-29 whole cell lysate at 20 µg

Lane 3:

U-2 OS whole cell lysate at 20 µg

Lane 4:

NCI-H460 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-DAAM1 antibody [EPR25376-223] - BSA and Azide free (AB307161)

This data was developed using ab307160, the same antibody clone in a different buffer formulation. Buffer/dilution concentration : 5% NFDM/TBST Exposure : Lanes 1,3 : 180 seconds, Lanes 2,4, and 5 : 92 seconds

All lanes:

Western blot - Anti-DAAM1 antibody [EPR25376-223] (<a href='/en-us/products/primary-antibodies/daam1-antibody-epr25376-223-ab307160'>ab307160</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Neuro-2a whole cell lysate at 20 µg

Lane 4:

C2C12 whole cell lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 123 kDa

false

Exposure time: 180s