AB169527

Anti-DAAM2 antibody [EPR10797(B]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal DAAM2 antibody. Suitable for WB and reacts with Human, Rat samples. Cited in 3 publications.

View Alternative Names

KIAA0381, DAAM2, Disheveled-associated activator of morphogenesis 2

1 Images

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Western blot - Anti-DAAM2 antibody [EPR10797(B] (AB169527)

All lanes:

Western blot - Anti-DAAM2 antibody [EPR10797(B] (ab169527) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

Fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 123 kDa

Observed band size: 123 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10797(B

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity. During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A. Also regulates non-canonical Wnt signaling pathway. Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery : affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation. Together with DAAM1, required for myocardial maturation and sarcomere assembly. Is a regulator of actin nucleation and elongation, filopodia formation and podocyte migration (PubMed : 33232676).

See full target information DAAM2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70992 PubMed40485623

2025

Integrated Analysis of Single-Cell and Bulk RNA-Sequencing Defines N7-Methylguanosine (m7G)-Mediated Modifications' Role in Prognosis and the Tumor Immune Microenvironment in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahua Liang,Mingjian Ma,Borui Xu,Cheng Zhong,Guangyan Zeng,Huijiao Lu,Jiaming Lai,Jiancong Chen

Nature 617:616-622 PubMed36972684

2023

Formin-mediated nuclear actin at androgen receptors promotes transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Knerr,Ralf Werner,Carsten Schwan,Hong Wang,Peter Gebhardt,Helga Grötsch,Almuth Caliebe,Malte Spielmann,Paul-Martin Holterhus,Robert Grosse,Nadine C Hornig

American journal of human genetics 107:1113-1128 PubMed33232676

2020

DAAM2 Variants Cause Nephrotic Syndrome via Actin Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ronen Schneider,Konstantin Deutsch,Gregory J Hoeprich,Jonathan Marquez,Tobias Hermle,Daniela A Braun,Steve Seltzsam,Thomas M Kitzler,Youying Mao,Florian Buerger,Amar J Majmundar,Ana C Onuchic-Whitford,Caroline M Kolvenbach,Luca Schierbaum,Sophia Schneider,Abdul A Halawi,Makiko Nakayama,Nina Mann,Dervla M Connaughton,Verena Klämbt,Matias Wagner,Korbinian M Riedhammer,Lutz Renders,Yoshichika Katsura,Dean Thumkeo,Neveen A Soliman,Shrikant Mane,Richard P Lifton,Shirlee Shril,Mustafa K Khokha,Julia Hoefele,Bruce L Goode,Friedhelm Hildebrandt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DAAM2 antibody [EPR10797(B]

Western blot - Anti-DAAM2 antibody [EPR10797(B] (AB169527)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (3)

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Product promise