AB111684

Anti-Dab1 antibody

Name: Anti-Dab1 antibody (ab111684) | Abcam
Brand: Abcam Limited
SKU: AB111684

(1 Review)

(5 Publications)

Rabbit Polyclonal Dab1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DAB1 aa 1-250.

View Alternative Names

Disabled homolog 1, DAB1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Dab1 antibody (AB111684)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dab1 antibody (AB111684)

HepG2 cells stained for DAB1 (green) using ab111684 at 1/500 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dab1 antibody (AB111684)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dab1 antibody (AB111684)

Paraffin embedded mouse duodenum tissue stained for DAB1 using ab111684 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Dab1 antibody (AB111684)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Dab1 antibody (ab111684) at 1/1000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Predicted band size: 64 kDa

false

Unknown

Western blot - Anti-Dab1 antibody (AB111684)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Dab1 antibody (ab111684) at 1/3000 dilution

All lanes:

Mouse brain cell lysate at 50 µg

Predicted band size: 57 kDa,60 kDa,61 kDa,62 kDa,63 kDa,64 kDa,68 kDa,69 kDa,70 kDa,71 kDa,73 kDa,74 kDa,77 kDa,79 kDa,80 kDa,84 kDa,85 kDa,87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DAB1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O75553

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab111684 is purified by antigen-affinity chromatography.

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signaling adapter of the reelin-mediated signaling pathway, which regulates the migration and differentiation of postmitotic neurons during brain development. Mediates intracellular transduction of Reelin signaling following reelin (RELN)-binding to its receptor : acts by docking proteins through its phosphotyrosine residues and PID domain.

See full target information DAB1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular neurosciences 112:103614 PubMed33845123

2021

Defective Reelin/Dab1 signaling pathways associated with disturbed hippocampus development of homozygous yotari mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nagisa Arimitsu,Yoshihisa Mizukami,Jun Shimizu,Kenji Takai,Tomoko Suzuki,Noboru Suzuki

Cell cycle (Georgetown, Tex.) 19:2793-2810 PubMed33064976

2020

Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of .

Applications

Unspecified application

Species

Unspecified reactive species

Lin Li,Jing Hao,Cheng-Quan Yan,He-Feng Wang,Bin Meng,Sheng-Yong Cai

Experimental neurology 320:112970 PubMed31185198

2019

Roles of Reelin/Disabled1 pathway on functional recovery of hemiplegic mice after neural cell transplantation; Reelin promotes migration toward motor cortex and maturation to motoneurons of neural grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Nagisa Arimitsu,Kenji Takai,Naruyoshi Fujiwara,Jun Shimizu,Yuji Ueda,Sueshige Wakisaka,Chieko Hirotsu,Masanori A Murayama,Tomoko Suzuki,Noboru Suzuki

PloS one 13:e0200268 PubMed30001399

2018

Reelin controls the positioning of brainstem serotonergic raphe neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Reham Shehabeldin,David Lutz,Meliha Karsak,Michael Frotscher,Kerstin Krieglstein,Ahmed Sharaf

Cell and tissue research 359:393-407 PubMed25418135

2014

Localization of reelin signaling pathway components in murine midbrain and striatum.

Applications

Unspecified application

Species

Mouse

Ahmed Sharaf,Belal Rahhal,Björn Spittau,Eleni Roussa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dab1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Dab1 antibody (AB111684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dab1 antibody (AB111684)

Western blot - Anti-Dab1 antibody (AB111684)

Western blot - Anti-Dab1 antibody (AB111684)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise