Anti-DACH1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DACH1 antibody (AB226176)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for DACH1 using ab226176 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining. Counterstain : Hematoxylin (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-DACH1 antibody (AB226176)

DACH1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226176 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226176 at 1 μg/ml.

Lane 1 : ab226176 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-DACH1 antibody (ab226176)

Predicted band size: 79 kDa

false

• WB

Supplier Data

Western blot - Anti-DACH1 antibody (AB226176)

All lanes:

Western blot - Anti-DACH1 antibody (ab226176) at 0.04 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 30s