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AB23836

Anti-DAD1 antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal DAD1 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DAD1.

View Alternative Names

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, Defender against cell death 1, DAD-1, DAD1

2 Images
Immunocytochemistry - Anti-DAD1 antibody (AB23836)
  • ICC

Unknown

Immunocytochemistry - Anti-DAD1 antibody (AB23836)

ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.

Western blot - Anti-DAD1 antibody (AB23836)
  • WB

Unknown

Western blot - Anti-DAD1 antibody (AB23836)

Lane 1:

Western blot - Anti-DAD1 antibody (ab23836) at 0.5 µg/mL

Lane 2:

Western blot - Anti-DAD1 antibody (ab23836) at 1 µg/mL

Lane 3:

Western blot - Anti-DAD1 antibody (ab23836) at 2 µg/mL

All lanes:

HepG2 whole cell lysate

Predicted band size: 12 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human DAD1. The exact immunogen used to generate this antibody is proprietary information.

P61803

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified by affinity chromatography.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 22467853, PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed : 22467853).
See full target information DAD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 289:32044-55 PubMed25301944

2014

The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides.

Applications

WB

Species

Unspecified reactive species

Yun Ding,Cosma D Dellisanti,Mi Hee Ko,Cynthia Czajkowski,Luigi Puglielli

Biochemical and biophysical research communications 387:381-6 PubMed19607806

2009

CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation.

Applications

WB

Species

Human

Thomas T Murooka,Ramtin Rahbar,Eleanor N Fish
View all publications
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