Anti-DAG1 antibody [IIH6C4]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunohistochemical analysis of paraffin-embedded sections human stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunohistochemical analysis of paraffin-embedded sections human skeletal muscle tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on human skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunohistochemical analysis of paraffin-embedded sections human pancreas tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membrane staining on human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunohistochemical analysis of paraffin-embedded sections rat stomach tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membrane staining on rat stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunohistochemical analysis of paraffin-embedded sections mouse kidney tissue labeling DAG1 with ab234587 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on rat skeletal muscle. The nuclear counter stain is DAPI (blue).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse skeletal muscle frozen sections labeling DAG1 with ab234587 at 1/100 dilution, followed by Goat Anti-Mouse (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on mouse skeletal muscle. The nuclear counter stain is DAPI (blue).

• WB

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Western blot - Anti-DAG1 antibody [IIH6C4] (AB234587)

Blocking buffer : 5% NFDM/TBST.

Lanes 1-3 : 20 seconds Lane 4 : 3 minutes.

Lanes 1-3 used the 6% gel and lane 4 used the 4-20% gradient gel.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 31566294 )

All lanes:

Western blot - Anti-DAG1 antibody [IIH6C4] (ab234587) at 1/5000 dilution

Lane 1:

Human skeletal muscle at 10 µg

Lane 2:

Human heart at 10 µg

Lane 3:

Rat skeletal muscle at 10 µg

Lane 4:

Mouse skeletal muscle at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 97 kDa

Observed band size: 120-150 kDa

false

• WB

Lab

Western blot - Anti-DAG1 antibody [IIH6C4] (AB234587)

ab234587 was shown to react with DAG1 in wild-type HAP1 cells in Western blot with loss of signal observed in a DAG1 knockout cell line. Wild-type HAP1 and DAG1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab234587 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-DAG1 antibody [IIH6C4] (ab234587) at 1/500 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

DAG1 knock-out HAP1 lysate at 20 µg

Observed band size: 94 kDa

false