Goat Polyclonal DAGLA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human DAGLA aa 1000 to C-terminus.
Images
Publications
- Nature communications 13:17832022Opposite physiological and pathological mTORC1-mediated roles of the CB1 receptor in regulating renal tubular function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiad Hinden,Majdoleen Ahmad,Sharleen Hamad,Alina Nemirovski,Gergő Szanda,Sandra Glasmacher,Aviram Kogot-Levin,Rinat Abramovitch,Bernard Thorens,Jürg Gertsch,Gil Leibowitz,Joseph TamPubMed 35379807
- Biological psychiatry 90:263-2742021cAMP Signaling-Mediated Phosphorylation of Diacylglycerol Lipase α Regulates Interaction With Ankyrin-G and Dendritic Spine Morphology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSehyoun Yoon,Kristoffer Myczek,Peter PenzesPubMed 34099188
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human DAGLA aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4D2
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.3-1 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Serine hydrolase that hydrolyzes arachidonic acid-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG) (PubMed:14610053, PubMed:26668358, PubMed:23502535). Preferentially hydrolyzes sn-1 fatty acids from diacylglycerols (DAG) that contain arachidonic acid (AA) esterified at the sn-2 position to biosynthesize 2-AG (PubMed:14610053, PubMed:26668358, PubMed:23502535). Has negligible activity against other lipids including monoacylglycerols and phospholipids (PubMed:14610053). Plays a key role in regulating 2-AG signaling in the central nervous system (CNS). Regulates 2-AG involved in retrograde suppression at central synapses. Supports axonal growth during development and adult neurogenesis. Plays a role for eCB signaling in the physiological regulation of anxiety and depressive behaviors. Regulates also neuroinflammatory responses in the brain, in particular, LPS-induced microglial activation (By similarity).
Alternative names
Diacylglycerol lipase-alpha, DAGL-alpha, DGL-alpha, Neural stem cell-derived dendrite regulator, Sn1-specific diacylglycerol lipase alpha, DAGLA, C11orf11, KIAA0659, NSDDR
Recommended products
Goat Polyclonal DAGLA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human DAGLA aa 1000 to C-terminus.
Alternative names
Diacylglycerol lipase-alpha, DAGL-alpha, DGL-alpha, Neural stem cell-derived dendrite regulator, Sn1-specific diacylglycerol lipase alpha, DAGLA, C11orf11, KIAA0659, NSDDR
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human DAGLA aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4D2
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-DAGLA antibody (ab81984)
Some minor background is detected and is blocked by the immunizing peptide.
Primary incubation for 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-DAGLA antibody (ab81984) at 0.3 µg/mL
All lanes: human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 125 kDa, 22 kDa, 55 kDa
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