AB81984

Anti-DAGLA antibody

Name: Anti-DAGLA antibody (ab81984) | Abcam
Brand: Abcam Limited
SKU: AB81984
Price: 565 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(9 Publications)

Goat Polyclonal DAGLA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human DAGLA aa 1000 to C-terminus.

View Alternative Names

C11orf11, KIAA0659, NSDDR, DAGLA, Diacylglycerol lipase-alpha, DAGL-alpha, DGL-alpha, Neural stem cell-derived dendrite regulator, Sn1-specific diacylglycerol lipase alpha

1 Images

Unknown

Western blot - Anti-DAGLA antibody (AB81984)

Some minor background is detected and is blocked by the immunizing peptide.

Primary incubation for 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-DAGLA antibody (ab81984) at 0.3 µg/mL

All lanes:

human liver lysate (in RIPA buffer) at 35 µg

Predicted band size: 114 kDa

Observed band size: 125 kDa,22 kDa,55 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DAGLA aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4D2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine hydrolase that hydrolyzes arachidonic acid-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG) (PubMed : 14610053, PubMed : 23502535, PubMed : 26668358). Preferentially hydrolyzes sn-1 fatty acids from diacylglycerols (DAG) that contain arachidonic acid (AA) esterified at the sn-2 position to biosynthesize 2-AG (PubMed : 14610053, PubMed : 23502535, PubMed : 26668358). Has negligible activity against other lipids including monoacylglycerols and phospholipids (PubMed : 14610053). Plays a key role in regulating 2-AG signaling in the central nervous system (CNS). Regulates 2-AG involved in retrograde suppression at central synapses. Supports axonal growth during development and adult neurogenesis. Plays a role for eCB signaling in the physiological regulation of anxiety and depressive behaviors. Also regulates neuroinflammatory responses in the brain, in particular, LPS-induced microglial activation (By similarity).

See full target information DAGLA

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 69:60-76 PubMed36780817

2023

Neuroimaging revealed long-lasting glucose metabolism changes to morphine withdrawal in rats pretreated with the cannabinoid agonist CP-55,940 during periadolescence.

Applications

Unspecified application

Species

Unspecified reactive species

N Lamanna-Rama,K S MacDowell,G López,J C Leza,M Desco,E Ambrosio,M L Soto-Montenegro

Nature communications 13:1783 PubMed35379807

2022

Opposite physiological and pathological mTORC1-mediated roles of the CB1 receptor in regulating renal tubular function.

Applications

Unspecified application

Species

Unspecified reactive species

Liad Hinden,Majdoleen Ahmad,Sharleen Hamad,Alina Nemirovski,Gergő Szanda,Sandra Glasmacher,Aviram Kogot-Levin,Rinat Abramovitch,Bernard Thorens,Jürg Gertsch,Gil Leibowitz,Joseph Tam

Biological psychiatry 90:263-274 PubMed34099188

2021

cAMP Signaling-Mediated Phosphorylation of Diacylglycerol Lipase α Regulates Interaction With Ankyrin-G and Dendritic Spine Morphology.

Applications

Unspecified application

Species

Unspecified reactive species

Sehyoun Yoon,Kristoffer Myczek,Peter Penzes

Addiction biology 26:e13007 PubMed33496035

2021

A role for the endocannabinoid enzymes monoacylglycerol and diacylglycerol lipases in cue-induced cocaine craving following prolonged abstinence.

Applications

Unspecified application

Species

Unspecified reactive species

Swarup Mitra,Pedro H Gobira,Craig T Werner,Jennifer A Martin,Madoka Iida,Shruthi A Thomas,Kyra Erias,Sophia Miracle,Charles Lafargue,Chunna An,David M Dietz

Journal of physiology and pharmacology : an offici 70: PubMed31889042

2019

Brain region-dependent changes in the expression of endocannabinoid-metabolizing enzymes in rats following antidepressant drugs.

Applications

Unspecified application

Species

Unspecified reactive species

I Smaga,D Gawlinski,J Brodowicz,M Filip

Pharmacology, biochemistry, and behavior 184:172738 PubMed31229467

2019

Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Shan Xue,Fen Xue,Quan-Rui Ma,Shi-Quan Wang,Ying Wang,Qing-Rong Tan,Hua-Ning Wang,Cui-Hong Zhou,Zheng-Wu Peng

Experimental cell research 367:112-118 PubMed29614312

2018

Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.

Applications

Species

Unspecified reactive species

Yasuhiko Okubo,Atsushi Kasamatsu,Masanobu Yamatoji,Kazuaki Fushimi,Takashi Ishigami,Toshihiro Shimizu,Hiroki Kasama,Masashi Shiiba,Hideki Tanzawa,Katsuhiro Uzawa

Neurotoxicity research 31:421-435 PubMed28247204

2017

Changes in the Brain Endocannabinoid System in Rat Models of Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Irena Smaga,Joanna Jastrzębska,Magdalena Zaniewska,Beata Bystrowska,Dawid Gawliński,Agata Faron-Górecka,Żaneta Broniowska,Joanna Miszkiel,Małgorzata Filip

Neuroscience 300:539-53 PubMed26037800

2015

An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction.

Applications

Species

Mouse

C R Hutch,C J Hillard,C Jia,C C Hegg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DAGLA antibody

Western blot - Anti-DAGLA antibody (AB81984)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise