Goat Polyclonal DAGLA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human DAGLA aa 1000 to C-terminus.
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Publications
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- Nature communications 13:17832022Opposite physiological and pathological mTORC1-mediated roles of the CB1 receptor in regulating renal tubular function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiad Hinden,Majdoleen Ahmad,Sharleen Hamad,Alina Nemirovski,Gergő Szanda,Sandra Glasmacher,Aviram Kogot-Levin,Rinat Abramovitch,Bernard Thorens,Jürg Gertsch,Gil Leibowitz,Joseph TamPubMed 35379807
- Biological psychiatry 90:263-2742021cAMP Signaling-Mediated Phosphorylation of Diacylglycerol Lipase α Regulates Interaction With Ankyrin-G and Dendritic Spine Morphology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSehyoun Yoon,Kristoffer Myczek,Peter PenzesPubMed 34099188
- Addiction biology 26:e130072021A role for the endocannabinoid enzymes monoacylglycerol and diacylglycerol lipases in cue-induced cocaine craving following prolonged abstinence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSwarup Mitra,Pedro H Gobira,Craig T Werner,Jennifer A Martin,Madoka Iida,Shruthi A Thomas,Kyra Erias,Sophia Miracle,Charles Lafargue,Chunna An,David M DietzPubMed 33496035
- Journal of physiology and pharmacology : an offici 70:2019Brain region-dependent changes in the expression of endocannabinoid-metabolizing enzymes in rats following antidepressant drugs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI Smaga et. al.PubMed 31889042
- Pharmacology, biochemistry, and behavior 184:1727382019Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan-Shan Xue et. al.PubMed 31229467
- Experimental cell research 367:112-1182018Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.
- Neurotoxicity research 31:421-4352017Changes in the Brain Endocannabinoid System in Rat Models of Depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrena Smaga,Joanna Jastrzębska,Magdalena Zaniewska,Beata Bystrowska,Dawid Gawliński,Agata Faron-Górecka,Żaneta Broniowska,Joanna Miszkiel,Małgorzata FilipPubMed 28247204
- Neuroscience 300:539-532015An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DAGLA aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4D2
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.3-1 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Serine hydrolase that hydrolyzes arachidonic acid-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG) (PubMed:14610053, PubMed:23502535, PubMed:26668358). Preferentially hydrolyzes sn-1 fatty acids from diacylglycerols (DAG) that contain arachidonic acid (AA) esterified at the sn-2 position to biosynthesize 2-AG (PubMed:14610053, PubMed:23502535, PubMed:26668358). Has negligible activity against other lipids including monoacylglycerols and phospholipids (PubMed:14610053). Plays a key role in regulating 2-AG signaling in the central nervous system (CNS). Regulates 2-AG involved in retrograde suppression at central synapses. Supports axonal growth during development and adult neurogenesis. Plays a role for eCB signaling in the physiological regulation of anxiety and depressive behaviors. Regulates also neuroinflammatory responses in the brain, in particular, LPS-induced microglial activation (By similarity).
Alternative names
C11orf11, KIAA0659, NSDDR, DAGLA, Diacylglycerol lipase-alpha, DAGL-alpha, DGL-alpha, Neural stem cell-derived dendrite regulator, Sn1-specific diacylglycerol lipase alpha
Recommended products
Goat Polyclonal DAGLA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human DAGLA aa 1000 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DAGLA aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4D2
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
Western blot - Anti-DAGLA antibody (ab81984)
Some minor background is detected and is blocked by the immunizing peptide.
Primary incubation for 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-DAGLA antibody (ab81984) at 0.3 µg/mL
All lanes: human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 125 kDa, 22 kDa, 55 kDa
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Product protocols
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