Anti-DAGLB antibody - C-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-DAGLB antibody - C-terminal (AB191159)

Detection of DAGLB in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab191159 at 6 μg/mg lysate for IP and at 0.4 μg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DAGLB antibody - C-terminal (ab191159)

Predicted band size: 73 kDa

false

• WB

Supplier Data

Western blot - Anti-DAGLB antibody - C-terminal (AB191159)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-DAGLB antibody - C-terminal (ab191159) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 73 kDa

true

Exposure time: 3min