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AB137623

Anti-DAK antibody

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(2 Publications)

Rabbit Polyclonal DAK antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TKFC aa 250-550.

View Alternative Names

DAK, TKFC, Triokinase/FMN cyclase, Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAK antibody (AB137623)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAK antibody (AB137623)

Immunohistochemical analysis of paraffin embedded Human lung SCC labelling DAK with ab137623 antibody at a dilution of 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-DAK antibody (AB137623)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DAK antibody (AB137623)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling DAK with ab137623 at 1/100 dilution. Picture at the bottom costained with Hoechst 33342.

Western blot - Anti-DAK antibody (AB137623)
  • WB

Unknown

Western blot - Anti-DAK antibody (AB137623)

7.5% SDS PAGE

All lanes:

Western blot - Anti-DAK antibody (ab137623) at 1/500 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TKFC aa 250-550. The exact immunogen used to generate this antibody is proprietary information.

Q3LXA3

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response (PubMed : 17600090).
See full target information TKFC

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112234 PubMed36897778

2023

The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Boehm,Victor Lam,Martina Schnolzer,Melanie Ott

Proteomics 22:e2100141 PubMed34932872

2022

Comprehensive kinomic study via a chemical proteomic approach reveals kinome reprogramming in hepatocellular carcinoma tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Wang,Xinzheng Wang,Xin Yang,Feng Liu,Jin Li,Weihua Li,Zhaofang Bai,Hongbo Wang,Jie Mao,Tingting Li,Kun He,Hongxia Wang
View all publications

Product promise

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