AB32056

Anti-DAP1 antibody [E59]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal DAP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

DAP1, DAP, Death-associated protein 1, DAP-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP1 antibody [E59] (AB32056)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP1 antibody [E59] (AB32056)

ab32056 at a 1/100 dilution staining human stomach carcinoma by immunohistochemistry Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-DAP1 antibody [E59] (AB32056)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DAP1 antibody [E59] (AB32056)

Immunofluorescent analysis of DAP1 expression in HeLa cells using 1/250 ab32056.

Unknown

Western blot - Anti-DAP1 antibody [E59] (AB32056)

All lanes:

Western blot - Anti-DAP1 antibody [E59] (ab32056) at 1/10000 dilution

All lanes:

NIH3T3 cell lysate

Predicted band size: 11 kDa

Observed band size: 11 kDa,23 kDa

false

Carrier free

Anti-DAP1 antibody [E59] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E59

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ribosome-binding protein involved in ribosome hibernation, a process during which ribosomes are stabilized in an inactive state and preserved from proteasomal degradation (By similarity). Acts via its association with eiF5a (EIF5A and EIF5A2) at the polypeptide exit tunnel of the ribosome, preventing mRNA translation (By similarity). Involved in ribosome hibernation in the mature oocyte by preventing mRNA translation, leading to ribosome inactivation (By similarity). Ribosomes, which are produced in large quantities during oogenesis, are stored and translationally repressed in the oocyte and early embryo (By similarity). Also acts as a negative regulator of autophagy (PubMed : 20537536). Involved in mediating interferon-gamma-induced cell death (PubMed : 7828849).

See full target information DAP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:106781 PubMed40837761

2025

regulates cell proliferation, migration, apoptosis and ferroptosis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Zu-Guo Yuan,Kai-Feng Zheng,Ke Chen

Journal of orthopaedic surgery and research 18:711 PubMed37735431

2023

DAP1 regulates osteoblast autophagy via the ATG16L1-LC3 axis in Graves' disease-induced osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingdong Gao,Zouxi Du,Qianqian Dong,Shan Su,Limin Tian

Genome biology 21:281 PubMed33213505

2020

Deep sequencing reveals a DAP1 regulatory haplotype that potentiates autoimmunity in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Prithvi Raj,Ran Song,Honglin Zhu,Linley Riediger,Dong-Jae Jun,Chaoying Liang,Carlos Arana,Bo Zhang,Yajing Gao,Benjamin E Wakeland,Igor Dozmorov,Jinchun Zhou,Jennifer A Kelly,Bernard R Lauwerys,Joel M Guthridge,Nancy J Olsen,Swapan K Nath,Chandrashekhar Pasare,Nicolai van Oers,Gary Gilkeson,Betty P Tsao,Patrick M Gaffney,Peter K Gregersen,Judith A James,Xiaoxia Zuo,David R Karp,Quan-Zhen Li,Edward K Wakeland

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DAP1 antibody [E59]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP1 antibody [E59] (AB32056)

Immunocytochemistry/ Immunofluorescence - Anti-DAP1 antibody [E59] (AB32056)

Western blot - Anti-DAP1 antibody [E59] (AB32056)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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