Rabbit Recombinant Monoclonal DAP13/NDUFA12 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Images
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img105997.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617)")
![Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunocytochemistry-immunofluorescence-img37608.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617)")
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img46468.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617)")
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img89831.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617)")
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img57628.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (AB192617)")
Publications
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- Nature communications 14:70692023Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Lin Zhou,Tao Zhang,Yun Xue,Chuan Yue,Yihui Pan,Pengyu Wang,Teng Yang,Meixia Li,Hu Zhou,Kan Ding,Jianhua Gan,Hongbin Ji,Cai-Guang YangPubMed 37923710
- Metabolism: clinical and experimental 115:1544312020A requirement for PAK1 to support mitochondrial function and maintain cellular redox balance via electron transport chain proteins to prevent β-cell apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiwon Ahn,Eunjin Oh,Erika M McCown,Xin Wang,Rajakrishnan Veluthakal,Debbie C ThurmondPubMed 33181191
- Cell research 29:579-5912019AIF-regulated oxidative phosphorylation supports lung cancer development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuan Rao et. al.PubMed 31133695
- DNA and cell biology 35:680-6902016Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/400 - 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/400 - 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Alternative names
DAP13, NDUFA12, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B17.2, NADH-ubiquinone oxidoreductase subunit B17.2, CI-B17.2, CIB17.2
Recommended products
Rabbit Recombinant Monoclonal DAP13/NDUFA12 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15867-28
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img105997.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/1000 dilution
Lane 1: mouse brain tissue lysate at 10 µg
Lanes 2 and 4: mouse heart tissue lysate at 10 µg
Lanes 3 and 5: mouse kidney tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunocytochemistry-immunofluorescence-img37608.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
Immunofluorescent analysis of HeLa cells (100% Methanol-fixed, 0.1% triton X-100-permealized) labeling DAP13/NDUFA12 with ab192617 at 1/100 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as secondary antibody. Cells were fixed with Counterstain: DAPI.
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img46468.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/5000 dilution
All lanes: Rat muscle tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img89831.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/5000 dilution
All lanes: Human fetal heart tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--western-blot-img57628.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/1000 dilution
Lane 1: Human fetal heart tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128345.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
Immunohistochemical analysis of paraffin-embedded Rat muscle tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73005.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10846.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128345.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73005.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-28-ab192617--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10846.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
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