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AB251148

Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DAP13/NDUFA12 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

DAP13, NDUFA12, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B17.2, NADH-ubiquinone oxidoreductase subunit B17.2, CI-B17.2, CIB17.2

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (100% Methanol-fixed, 0.1% triton X-100-permealized) labeling DAP13/NDUFA12 with ab192617 at 1/100 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with Counterstain : DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat muscle tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • WB

Unknown

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (<a href='/en-us/products/primary-antibodies/dap13-ndufa12-antibody-epr15867-28-ab192617'>ab192617</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 17 kDa

false

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • WB

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (<a href='/en-us/products/primary-antibodies/dap13-ndufa12-antibody-epr15867-28-ab192617'>ab192617</a>) at 1/5000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 17 kDa

false

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • WB

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (<a href='/en-us/products/primary-antibodies/dap13-ndufa12-antibody-epr15867-28-ab192617'>ab192617</a>) at 1/1000 dilution

Lane 1:

mouse brain tissue lysate at 10 µg

Lanes 2 and 4:

mouse heart tissue lysate at 10 µg

Lanes 3 and 5:

mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 17 kDa

false

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)
  • WB

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] - BSA and Azide free (AB251148)

This data was developed using ab192617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (<a href='/en-us/products/primary-antibodies/dap13-ndufa12-antibody-epr15867-28-ab192617'>ab192617</a>) at 1/5000 dilution

All lanes:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15867-28

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab251148 is the carrier-free version of ab192617.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFA12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com